HPLC Method for Determination of Atenolol in Human Plasma and Application to a Pharmacokinetic Study in Turkey
This paper describes a high-performance liquid chromatography method for the determination of atenolol in human plasma. Atenolol and the internal standard, metoprolol, were extracted from plasma by using a liquid-liquid extraction method. The method was developed on an Ace C18 reverse-phase column u...
Gespeichert in:
| Veröffentlicht in: | Journal of chromatographic science 2012-11, Vol.50 (10), p.914-919 |
|---|---|
| Hauptverfasser: | , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 919 |
|---|---|
| container_issue | 10 |
| container_start_page | 914 |
| container_title | Journal of chromatographic science |
| container_volume | 50 |
| creator | Yilmaz, Bilal Arslan, Sakir Asci, Ali |
| description | This paper describes a high-performance liquid chromatography method for the determination of atenolol in human plasma. Atenolol and the internal standard, metoprolol, were extracted from plasma by using a liquid-liquid extraction method. The method was developed on an Ace C18 reverse-phase column using a mobile phase of methanol-water (50:50, v/v) containing 0.1% trifluoroacetic acid. The calibration curve was linear within the concentration range of 5-150 ng/mL. Intra-day and inter-day precision values for atenolol in plasma were less than 6.1, and accuracy (relative error) was better than 5.5%. The mean recovery of atenolol was 98.4% for plasma. The limits of detection and quantification of atenolol were 1.5 and 5 ng/mL, respectively. Also, this assay was successfully applied to six patients with hypertension who had been given an oral tablet of 50 mg atenolol. |
| doi_str_mv | 10.1093/chromsci/bms090 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_1786149238</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><oup_id>10.1093/chromsci/bms090</oup_id><sourcerecordid>1786149238</sourcerecordid><originalsourceid>FETCH-LOGICAL-c436t-60c203e6c2938ae592eb5ba0965f8486a828fc93e73ce7bcf0358aabeeaabc0f3</originalsourceid><addsrcrecordid>eNqF0U2r1DAUBuAgine8unYn2Qgi1MlH0ybLYfwYYcQBr-CunGZOmXjbpCbpYv69HTpXl3eTEHjOGzgvIa85-8CZkWt7imFI1q3bITHDnpAVN6UqhDS_npIVY4IXpqrUDXmR0u_Lk2v1nNwIUXNdl3pF_O6w39JvmE_hSLsQ6UfMGAfnIbvgaejoJqMPfeip83Q3DeDpoYc0AAV_pJtx7J1dbA4U6OEEcQAb7p3H7Cz9kafj-TJ6N8V7PL8kzzroE7663rfk5-dPd9tdsf_-5et2sy9sKatcVMwKJrGywkgNqIzAVrXATKU6XeoKtNCdNRJrabFubcek0gAt4nxY1slb8m7JHWP4M2HKzeCSxb4Hj2FKDa91xUsjpH6ccqGEmpfIZrpeqI0hpYhdM0Y3QDw3nDWXPpqHPpqlj3nizTV8agc8_vMPBczg7RVAstB3Ebx16b-rlJFKXoLeLy5M46O__gURWaWI</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1125256650</pqid></control><display><type>article</type><title>HPLC Method for Determination of Atenolol in Human Plasma and Application to a Pharmacokinetic Study in Turkey</title><source>Oxford University Press Journals All Titles (1996-Current)</source><source>MEDLINE</source><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><source>Alma/SFX Local Collection</source><source>Free Full-Text Journals in Chemistry</source><creator>Yilmaz, Bilal ; Arslan, Sakir ; Asci, Ali</creator><creatorcontrib>Yilmaz, Bilal ; Arslan, Sakir ; Asci, Ali</creatorcontrib><description>This paper describes a high-performance liquid chromatography method for the determination of atenolol in human plasma. Atenolol and the internal standard, metoprolol, were extracted from plasma by using a liquid-liquid extraction method. The method was developed on an Ace C18 reverse-phase column using a mobile phase of methanol-water (50:50, v/v) containing 0.1% trifluoroacetic acid. The calibration curve was linear within the concentration range of 5-150 ng/mL. Intra-day and inter-day precision values for atenolol in plasma were less than 6.1, and accuracy (relative error) was better than 5.5%. The mean recovery of atenolol was 98.4% for plasma. The limits of detection and quantification of atenolol were 1.5 and 5 ng/mL, respectively. Also, this assay was successfully applied to six patients with hypertension who had been given an oral tablet of 50 mg atenolol.</description><identifier>ISSN: 0021-9665</identifier><identifier>EISSN: 1945-239X</identifier><identifier>DOI: 10.1093/chromsci/bms090</identifier><identifier>PMID: 22718748</identifier><identifier>CODEN: JCHSBZ</identifier><language>eng</language><publisher>Niles, IL: Oxford University Press</publisher><subject>Administration, Oral ; Analysis ; Antihypertensive Agents - blood ; Antihypertensive Agents - chemistry ; Antihypertensive Agents - pharmacokinetics ; Atenolol - blood ; Atenolol - chemistry ; Atenolol - pharmacokinetics ; Biological and medical sciences ; Calibration ; Chromatography ; Chromatography, High Pressure Liquid - methods ; Drug Stability ; General pharmacology ; Human ; Humans ; Hypertension ; Hypertension - blood ; Hypertension - drug therapy ; Least-Squares Analysis ; Limit of Detection ; Liquid chromatography ; Liquid-liquid extraction ; Male ; Medical sciences ; Metoprolol - analysis ; Pharmacology. Drug treatments ; Reproducibility of Results ; Tablets ; Turkey</subject><ispartof>Journal of chromatographic science, 2012-11, Vol.50 (10), p.914-919</ispartof><rights>The Author [2012]. Published by Oxford University Press. All rights reserved. For Permissions, please email: journals.permissions@oup.com 2012</rights><rights>2014 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c436t-60c203e6c2938ae592eb5ba0965f8486a828fc93e73ce7bcf0358aabeeaabc0f3</citedby><cites>FETCH-LOGICAL-c436t-60c203e6c2938ae592eb5ba0965f8486a828fc93e73ce7bcf0358aabeeaabc0f3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,1578,27901,27902</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=26593530$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/22718748$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Yilmaz, Bilal</creatorcontrib><creatorcontrib>Arslan, Sakir</creatorcontrib><creatorcontrib>Asci, Ali</creatorcontrib><title>HPLC Method for Determination of Atenolol in Human Plasma and Application to a Pharmacokinetic Study in Turkey</title><title>Journal of chromatographic science</title><addtitle>J Chromatogr Sci</addtitle><description>This paper describes a high-performance liquid chromatography method for the determination of atenolol in human plasma. Atenolol and the internal standard, metoprolol, were extracted from plasma by using a liquid-liquid extraction method. The method was developed on an Ace C18 reverse-phase column using a mobile phase of methanol-water (50:50, v/v) containing 0.1% trifluoroacetic acid. The calibration curve was linear within the concentration range of 5-150 ng/mL. Intra-day and inter-day precision values for atenolol in plasma were less than 6.1, and accuracy (relative error) was better than 5.5%. The mean recovery of atenolol was 98.4% for plasma. The limits of detection and quantification of atenolol were 1.5 and 5 ng/mL, respectively. Also, this assay was successfully applied to six patients with hypertension who had been given an oral tablet of 50 mg atenolol.</description><subject>Administration, Oral</subject><subject>Analysis</subject><subject>Antihypertensive Agents - blood</subject><subject>Antihypertensive Agents - chemistry</subject><subject>Antihypertensive Agents - pharmacokinetics</subject><subject>Atenolol - blood</subject><subject>Atenolol - chemistry</subject><subject>Atenolol - pharmacokinetics</subject><subject>Biological and medical sciences</subject><subject>Calibration</subject><subject>Chromatography</subject><subject>Chromatography, High Pressure Liquid - methods</subject><subject>Drug Stability</subject><subject>General pharmacology</subject><subject>Human</subject><subject>Humans</subject><subject>Hypertension</subject><subject>Hypertension - blood</subject><subject>Hypertension - drug therapy</subject><subject>Least-Squares Analysis</subject><subject>Limit of Detection</subject><subject>Liquid chromatography</subject><subject>Liquid-liquid extraction</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Metoprolol - analysis</subject><subject>Pharmacology. Drug treatments</subject><subject>Reproducibility of Results</subject><subject>Tablets</subject><subject>Turkey</subject><issn>0021-9665</issn><issn>1945-239X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqF0U2r1DAUBuAgine8unYn2Qgi1MlH0ybLYfwYYcQBr-CunGZOmXjbpCbpYv69HTpXl3eTEHjOGzgvIa85-8CZkWt7imFI1q3bITHDnpAVN6UqhDS_npIVY4IXpqrUDXmR0u_Lk2v1nNwIUXNdl3pF_O6w39JvmE_hSLsQ6UfMGAfnIbvgaejoJqMPfeip83Q3DeDpoYc0AAV_pJtx7J1dbA4U6OEEcQAb7p3H7Cz9kafj-TJ6N8V7PL8kzzroE7663rfk5-dPd9tdsf_-5et2sy9sKatcVMwKJrGywkgNqIzAVrXATKU6XeoKtNCdNRJrabFubcek0gAt4nxY1slb8m7JHWP4M2HKzeCSxb4Hj2FKDa91xUsjpH6ccqGEmpfIZrpeqI0hpYhdM0Y3QDw3nDWXPpqHPpqlj3nizTV8agc8_vMPBczg7RVAstB3Ebx16b-rlJFKXoLeLy5M46O__gURWaWI</recordid><startdate>20121101</startdate><enddate>20121101</enddate><creator>Yilmaz, Bilal</creator><creator>Arslan, Sakir</creator><creator>Asci, Ali</creator><general>Oxford University Press</general><general>Preston Publications</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7SR</scope><scope>8BQ</scope><scope>8FD</scope><scope>JG9</scope></search><sort><creationdate>20121101</creationdate><title>HPLC Method for Determination of Atenolol in Human Plasma and Application to a Pharmacokinetic Study in Turkey</title><author>Yilmaz, Bilal ; Arslan, Sakir ; Asci, Ali</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c436t-60c203e6c2938ae592eb5ba0965f8486a828fc93e73ce7bcf0358aabeeaabc0f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>Administration, Oral</topic><topic>Analysis</topic><topic>Antihypertensive Agents - blood</topic><topic>Antihypertensive Agents - chemistry</topic><topic>Antihypertensive Agents - pharmacokinetics</topic><topic>Atenolol - blood</topic><topic>Atenolol - chemistry</topic><topic>Atenolol - pharmacokinetics</topic><topic>Biological and medical sciences</topic><topic>Calibration</topic><topic>Chromatography</topic><topic>Chromatography, High Pressure Liquid - methods</topic><topic>Drug Stability</topic><topic>General pharmacology</topic><topic>Human</topic><topic>Humans</topic><topic>Hypertension</topic><topic>Hypertension - blood</topic><topic>Hypertension - drug therapy</topic><topic>Least-Squares Analysis</topic><topic>Limit of Detection</topic><topic>Liquid chromatography</topic><topic>Liquid-liquid extraction</topic><topic>Male</topic><topic>Medical sciences</topic><topic>Metoprolol - analysis</topic><topic>Pharmacology. Drug treatments</topic><topic>Reproducibility of Results</topic><topic>Tablets</topic><topic>Turkey</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Yilmaz, Bilal</creatorcontrib><creatorcontrib>Arslan, Sakir</creatorcontrib><creatorcontrib>Asci, Ali</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Engineered Materials Abstracts</collection><collection>METADEX</collection><collection>Technology Research Database</collection><collection>Materials Research Database</collection><jtitle>Journal of chromatographic science</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Yilmaz, Bilal</au><au>Arslan, Sakir</au><au>Asci, Ali</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>HPLC Method for Determination of Atenolol in Human Plasma and Application to a Pharmacokinetic Study in Turkey</atitle><jtitle>Journal of chromatographic science</jtitle><addtitle>J Chromatogr Sci</addtitle><date>2012-11-01</date><risdate>2012</risdate><volume>50</volume><issue>10</issue><spage>914</spage><epage>919</epage><pages>914-919</pages><issn>0021-9665</issn><eissn>1945-239X</eissn><coden>JCHSBZ</coden><abstract>This paper describes a high-performance liquid chromatography method for the determination of atenolol in human plasma. Atenolol and the internal standard, metoprolol, were extracted from plasma by using a liquid-liquid extraction method. The method was developed on an Ace C18 reverse-phase column using a mobile phase of methanol-water (50:50, v/v) containing 0.1% trifluoroacetic acid. The calibration curve was linear within the concentration range of 5-150 ng/mL. Intra-day and inter-day precision values for atenolol in plasma were less than 6.1, and accuracy (relative error) was better than 5.5%. The mean recovery of atenolol was 98.4% for plasma. The limits of detection and quantification of atenolol were 1.5 and 5 ng/mL, respectively. Also, this assay was successfully applied to six patients with hypertension who had been given an oral tablet of 50 mg atenolol.</abstract><cop>Niles, IL</cop><pub>Oxford University Press</pub><pmid>22718748</pmid><doi>10.1093/chromsci/bms090</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0021-9665 |
| ispartof | Journal of chromatographic science, 2012-11, Vol.50 (10), p.914-919 |
| issn | 0021-9665 1945-239X |
| language | eng |
| recordid | cdi_proquest_miscellaneous_1786149238 |
| source | Oxford University Press Journals All Titles (1996-Current); MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Alma/SFX Local Collection; Free Full-Text Journals in Chemistry |
| subjects | Administration, Oral Analysis Antihypertensive Agents - blood Antihypertensive Agents - chemistry Antihypertensive Agents - pharmacokinetics Atenolol - blood Atenolol - chemistry Atenolol - pharmacokinetics Biological and medical sciences Calibration Chromatography Chromatography, High Pressure Liquid - methods Drug Stability General pharmacology Human Humans Hypertension Hypertension - blood Hypertension - drug therapy Least-Squares Analysis Limit of Detection Liquid chromatography Liquid-liquid extraction Male Medical sciences Metoprolol - analysis Pharmacology. Drug treatments Reproducibility of Results Tablets Turkey |
| title | HPLC Method for Determination of Atenolol in Human Plasma and Application to a Pharmacokinetic Study in Turkey |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-11T11%3A42%3A05IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=HPLC%20Method%20for%20Determination%20of%20Atenolol%20in%20Human%20Plasma%20and%20Application%20to%20a%20Pharmacokinetic%20Study%20in%20Turkey&rft.jtitle=Journal%20of%20chromatographic%20science&rft.au=Yilmaz,%20Bilal&rft.date=2012-11-01&rft.volume=50&rft.issue=10&rft.spage=914&rft.epage=919&rft.pages=914-919&rft.issn=0021-9665&rft.eissn=1945-239X&rft.coden=JCHSBZ&rft_id=info:doi/10.1093/chromsci/bms090&rft_dat=%3Cproquest_cross%3E1786149238%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1125256650&rft_id=info:pmid/22718748&rft_oup_id=10.1093/chromsci/bms090&rfr_iscdi=true |