Production of cyclohexanone monooxygenase from Acinetobacter calcoaceticus for large scale Baeyer-Villiger monooxygenase reactions

Cyclohexanone monooxygenase was produced from Acinetobacter calcoaceticus grown on a medium containing both glutamate (30 g l^sup -1^) and cyclohexanol (1 g l^sup -1^). Productivity was increased to 650 U l^sup -1^, an order of magnitude greater than previous production methods, thereby enhancing th...

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Veröffentlicht in:	Biotechnology letters 2001-03, Vol.23 (5), p.385-388
Hauptverfasser:	BARCLAY, Scott S, WOODLEY, John M, LILLY, Malcolm D, SPARGO, Peter L, PETTMAN, Alan J
Format:	Artikel
Sprache:	eng
Schlagworte:	Acinetobacter calcoaceticus Bacteria Baeyer-Villiger monooxygenase Biological and medical sciences Biotechnology Chemical reactions Enzyme engineering Enzymes Fermentation Fundamental and applied biological sciences. Psychology Methods. Procedures. Technologies Production of selected enzymes
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Zusammenfassung:	Cyclohexanone monooxygenase was produced from Acinetobacter calcoaceticus grown on a medium containing both glutamate (30 g l^sup -1^) and cyclohexanol (1 g l^sup -1^). Productivity was increased to 650 U l^sup -1^, an order of magnitude greater than previous production methods, thereby enhancing the potential commercial utility of this enzyme.[PUBLICATION ABSTRACT]
ISSN:	0141-5492 1573-6776
DOI:	10.1023/A:1005610815727