Cloning, analysis and expression of the gene for β-glycosidase of Thermus caldophilus GK24 and properties of the enzyme

The gene (bglT) encoding Thermus caldophilus GK24 beta -glycosidase (Tca beta -glycosidase) was cloned and sequenced. The gene contains an open reading frame encoding 431 amino acids with a M sub(r) of 48 658 Da. The bglT gene was expressed under the control of tac promoter on a high-copy plasmid in E. coli. The recombinant Tca beta -glycosidase was purified 41.5-fold with a 59% yield and a specific activity of 83 U mg super(-1) protein.