Development of loop-mediated isothermal amplification assay for rapid screening of fungal contamination in pepper and paprika powder

Development of loop-mediated isothermal amplification assay for rapid screening of fungal contamination in pepper and paprika powder

Fungi cause hazards such as mildew, spoilage, and mycotoxins, so the Chinese government has formulated maximum level limits (MLs) for fungi in spices. However, the current standard culture-based detection method is time-consuming (5-7 days) and unsuitable for the rapid on-site screening of fungal co...

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Veröffentlicht in:Quality assurance and safety of crops & food 2015-01, Vol.7 (2), p.97-102
Hauptverfasser: Zhang, W.W, Jin, Y, Ye, Z.M, Wang, S.Y, Solfrizzo, M, Xu, X, Pei, X.F
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Sprache:eng
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Zusammenfassung:Fungi cause hazards such as mildew, spoilage, and mycotoxins, so the Chinese government has formulated maximum level limits (MLs) for fungi in spices. However, the current standard culture-based detection method is time-consuming (5-7 days) and unsuitable for the rapid on-site screening of fungal contamination to facilitate immediate control measures. Therefore, we developed a loop-mediated isothermal amplification (LAMP) assay for the rapid (
ISSN:1757-8361
1757-837X
DOI:10.3920/QAS2013.0266