Osteoinductive potential of biocomposite cylinders impregnated with Glycyrrhiza glabra for bone tissue engineering

Biocomposite cylinders containing biphasic calcium phosphate (BCP), collagen (CS), and an extract of Glycyrrhiza glabra (GG) were prepared and characterized for their physicochemical properties. Incorporation of BCP and GG enhanced the mechanical properties of the CS. The biomimetic mineralization capacity of CS–BCP–GG biocomposite cylinders was evaluated in a simulated body fluid solution. CS–BCP–GG biocomposite cylinders show over 80% viability in NIH-3T3 and SaOS-2 cell lines, compared to CS and CS–BCP. In vivo studies were performed using zebrafish embryos to find out the toxicity level of CS isolated from chrome shavings.