Screening for gestagens in kidney fat using accelerated solvent extraction and liquid chromatography electrospray tandem mass spectrometry

A screening method has been developed for the determination of various anabolic steroids in kidney fat. Fat samples are extracted and steroids are trapped “on-line” during accelerated solvent extraction (ASE). Following this initial extraction samples are further purified with C18 solid-phase extrac...

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Veröffentlicht in:	Analytica chimica acta 2003-04, Vol.483 (1), p.51-59
Hauptverfasser:	Hooijerink, H, van Bennekom, E.O, Nielen, M.W.F
Format:	Artikel
Sprache:	eng
Schlagworte:	Accelerated solvent extraction Analytical chemistry Animal productions Biological and medical sciences Chemistry Chromatographic methods and physical methods associated with chromatography Exact sciences and technology Fundamental and applied biological sciences. Psychology Gestagens Kidney fat Liquid chromatography Other chromatographic methods Spectrometric and optical methods Tandem mass spectrometry Terrestrial animal productions Vertebrates
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Zusammenfassung:	A screening method has been developed for the determination of various anabolic steroids in kidney fat. Fat samples are extracted and steroids are trapped “on-line” during accelerated solvent extraction (ASE). Following this initial extraction samples are further purified with C18 solid-phase extraction (SPE). Compounds were finally analyzed by liquid chromatography (LC) coupled with tandem mass spectrometry (MS). The method was validated using blank kidney fat and fat samples fortified at 2 ng g −1. The detection capability (CCβ) was
ISSN:	0003-2670 1873-4324
DOI:	10.1016/S0003-2670(02)01231-X