A colorimetric assay for gellan elaborated by Sphingomonas paucimobilis ATCC 31461

A colorimetric assay involving the dye toluidine blue O was developed to determine the concentration of the microbial heteropolysaccharide gellan elaborated by Sphingomonas paucimobilis ATCC 31461. Colour formation was linear up to a concentration of 0.7 mg/ml. The concentration of gellan produced i...

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Veröffentlicht in:	World journal of microbiology & biotechnology 2000-01, Vol.16 (6), p.529-531
Hauptverfasser:	WEST, T. P, STROHFUS, B, SANTIAGO, M. F
Format:	Artikel
Sprache:	eng
Schlagworte:	Biological and medical sciences Biotechnology Fundamental and applied biological sciences. Psychology gellan gravimetry Methods. Procedures. Technologies Microbiology Others Sphingomonas paucimobilis toluidine blue Various methods and equipments
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Zusammenfassung:	A colorimetric assay involving the dye toluidine blue O was developed to determine the concentration of the microbial heteropolysaccharide gellan elaborated by Sphingomonas paucimobilis ATCC 31461. Colour formation was linear up to a concentration of 0.7 mg/ml. The concentration of gellan produced in S. paucimobilis cultures was quantitated using this colorimetric dye-binding assay as well as the currently utilized gravimetric procedure, and comparable results were observed.[PUBLICATION ABSTRACT]
ISSN:	0959-3993 1573-0972
DOI:	10.1023/A:1008935031777