Synthesis of pentylferulate by a feruloyl esterase from Aspergillus niger using water-in-oil microemulsions

Pentylferulate synthesis was achieved at high yields (50-60%) with Aspergillus niger feruloyl esterase using a water-in-oil microemulsion system. The initial rate of synthesis decreased by 15-20% when the water content of the microemulsion was increased from 1.8 to 2.4% (v/v), although a concomitant decrease in conversion was not observed. The enzyme stability was significantly higher in the microemulsion than in an aqueous solution.[PUBLICATION ABSTRACT]