Expression of recombinant organophosphorus hydrolase in the original producer of the enzyme, Sphingobium fuliginis ATCC 27551

The plasmid encoding His-tagged organophosphorus hydrolase (OPH) cloned from Sphingobium fuliginis was modified to be transferred back to this bacterium. The replication function of S. amiense plasmid was inserted at downstream of OPH gene, and S. fuliginis was transformed with this plasmid. The tra...

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Veröffentlicht in:	Bioscience, biotechnology, and biochemistry biotechnology, and biochemistry, 2016-05, Vol.80 (5), p.1024-1026
Hauptverfasser:	Nakayama, Kosuke, Ohmori, Takeshi, Ishikawa, Satoshi, Iwata, Natsumi, Seto, Yasuo, Kawahara, Kazuyoshi
Format:	Artikel
Sprache:	eng
Schlagworte:	Aryldialkylphosphatase - genetics Aryldialkylphosphatase - metabolism Bacterial Proteins - genetics Bacterial Proteins - metabolism Biodegradation, Environmental DNA Replication DNA, Bacterial - genetics DNA, Bacterial - metabolism Escherichia coli - enzymology Escherichia coli - genetics Gene Expression Histidine - genetics Histidine - metabolism Humans nerve agent Nerve Agents - metabolism Oligopeptides - genetics Oligopeptides - metabolism Organophosphorus Compounds - metabolism organophosphorus hydrolase paraoxon Plasmids - chemistry Plasmids - metabolism Recombinant Fusion Proteins - genetics Recombinant Fusion Proteins - metabolism Sphingobium amiense Sphingobium fuliginis Sphingomonadaceae - enzymology Sphingomonadaceae - genetics Transformation, Bacterial
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Zusammenfassung:	The plasmid encoding His-tagged organophosphorus hydrolase (OPH) cloned from Sphingobium fuliginis was modified to be transferred back to this bacterium. The replication function of S. amiense plasmid was inserted at downstream of OPH gene, and S. fuliginis was transformed with this plasmid. The transformant produced larger amount of active OPH with His-tag than E. coli.
ISSN:	0916-8451 1347-6947
DOI:	10.1080/09168451.2015.1123606