Optimisation of the dehydrogenase assay for measurement of indigenous microbial activity in beach sediments contaminated with petroleum

A dehydrogenase enzyme assay has been developed to measure microbial activity in petroleum-contaminated beach sediments. The assay, using 2-(p-iodophenyl)-3-(p-nitrophenyl)-5-phenyltetrazoliumchloride (INT) as the substrate provides a reliable measurement of overall indigenous microbiological activi...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Veröffentlicht in:	Biotechnology letters 2001-02, Vol.23 (3), p.227-230
Hauptverfasser:	MATHEW, M, OBBARD, J. P
Format:	Artikel
Sprache:	eng
Schlagworte:	Applied sciences Beaches Biodegradation Biodegradation of pollutants Biological and medical sciences Biotechnology Contaminated sediments Decontamination. Miscellaneous Dehydrogenase Environment and pollution Exact sciences and technology Fundamental and applied biological sciences. Psychology Industrial applications and implications. Economical aspects Microbial activity petroleum Petroleum hydrocarbons Pollution Sediment pollution Soil and sediments pollution Soils
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Beschreibung
Zusammenfassung:	A dehydrogenase enzyme assay has been developed to measure microbial activity in petroleum-contaminated beach sediments. The assay, using 2-(p-iodophenyl)-3-(p-nitrophenyl)-5-phenyltetrazoliumchloride (INT) as the substrate provides a reliable measurement of overall indigenous microbiological activity in beach sediments containing 7% (w/w) oil. The method has been effectively used in field studies (Mathew et al. 1999) to monitor microbial activity in conjunction with the biodegradation of petroleum hydrocarbons.[PUBLICATION ABSTRACT]
ISSN:	0141-5492 1573-6776
DOI:	10.1023/A:1005613406948