Ultrafine particulate matter and high-level benzene urban air pollution in relation to oxidative DNA damage
Air pollution, containing high-level of ultrafine particles (UFP) and benzene, is a prominent environmental health problem in many cities of the World. We investigated the level of oxidative DNA damage in mononuclear blood cells (MNBC) by the comet assay as DNA strand breaks (SB) and formamidopyrimi...
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Veröffentlicht in: | Carcinogenesis (New York) 2005-03, Vol.26 (3), p.613-620 |
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creator | Avogbe, Patrice H. Ayi-Fanou, Lucie Autrup, Herman Loft, Steffen Fayomi, Benjamin Sanni, Ambaliou Vinzents, Peter Møller, Peter |
description | Air pollution, containing high-level of ultrafine particles (UFP) and benzene, is a prominent environmental health problem in many cities of the World. We investigated the level of oxidative DNA damage in mononuclear blood cells (MNBC) by the comet assay as DNA strand breaks (SB) and formamidopyrimidine DNA glycosylase (FPG) sensitive sites in residents from three urban locations in Cotonou, Benin (taxi-moto drivers, subjects living near roads with intense traffic and suburban residents) and rural residents. Exposure was characterized by urinary excretion of S-phenylmercapturic acid (S-PMA), a biomarker of benzene exposure, and by ambient UFP. There were clear stepwise gradients with respect to ambient UFP, S-PMA excretion and oxidative DNA damage with rural subjects < suburban subjects < residents living near highly trafficed roads |
doi_str_mv | 10.1093/carcin/bgh353 |
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We investigated the level of oxidative DNA damage in mononuclear blood cells (MNBC) by the comet assay as DNA strand breaks (SB) and formamidopyrimidine DNA glycosylase (FPG) sensitive sites in residents from three urban locations in Cotonou, Benin (taxi-moto drivers, subjects living near roads with intense traffic and suburban residents) and rural residents. Exposure was characterized by urinary excretion of S-phenylmercapturic acid (S-PMA), a biomarker of benzene exposure, and by ambient UFP. There were clear stepwise gradients with respect to ambient UFP, S-PMA excretion and oxidative DNA damage with rural subjects < suburban subjects < residents living near highly trafficed roads<taxi-moto drivers. Polymorphisms in glutathione peroxidase (GPX), NAD(P)H:quinone oxidoreductase 1 (NQO1) and glutathione S-transferase (GST) genes were assessed for effect modification. Subjects with GSTT1 null genotype had lower urinary S-PMA excretion than subjects carrying the plus genotype. Urinary S-PMA excretion correlated with SB (R = 0.17) and FPG sites (R = 0.25) in MNBC. The correlation between S-PMA and SB was strongest in subjects with NQO1*1/*2 and *2/*2 genotypes (R = 0.37), and between S-PMA and FPG sensitive sites in subjects with the GSTP1*B/*B genotype (R = 0.39). In conclusion, this study shows that urban air with high levels of benzene and UFP is associated with elevated levels of SB and FPG sites in MNBC, and that NQO1 and GST genes may modulate the effect.</description><identifier>ISSN: 0143-3334</identifier><identifier>ISSN: 1460-2180</identifier><identifier>EISSN: 1460-2180</identifier><identifier>DOI: 10.1093/carcin/bgh353</identifier><identifier>PMID: 15591089</identifier><identifier>CODEN: CRNGDP</identifier><language>eng</language><publisher>Oxford: Oxford University Press</publisher><subject>8 dihydro-2′-deoxyguanosine ; 8-oxo-7 ; 8-oxodG ; Air ; Air Pollutants - analysis ; Air Pollutants - toxicity ; Benzene - analysis ; Benzene - toxicity ; Biological and medical sciences ; Biomarkers - analysis ; Carcinogenesis, carcinogens and anticarcinogens ; DNA Damage ; Environmental pollutants toxicology ; formamidopyrimidine DNA glycosylase ; FPG ; general linear model ; GLM ; glutathione peroxidase ; glutathione S-transferase ; GPX ; GST ; Humans ; least statistical difference ; LSD ; Male ; Medical sciences ; MNBC ; mononuclear blood cells ; NAD(P)H:quinone oxidoreductase 1 ; NQO1 ; Oxidative Stress ; PAH ; Particle Size ; particulate matter ; polyaromatic hydrocarbons ; reactive oxygen species ; ROS ; S-phenylmercapturic acid ; S-PMA ; strand breaks ; Toxicology ; Tumors ; UFP ; ultrafine particles ; Urban Health</subject><ispartof>Carcinogenesis (New York), 2005-03, Vol.26 (3), p.613-620</ispartof><rights>2005 INIST-CNRS</rights><rights>Copyright Oxford University Press(England) Mar 01, 2005</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c456t-eac03fa9ac4b240d59a7c662eddc6d5cc69b00e7d014f46c4c8de2f56d185f9b3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=16599735$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15591089$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Avogbe, Patrice H.</creatorcontrib><creatorcontrib>Ayi-Fanou, Lucie</creatorcontrib><creatorcontrib>Autrup, Herman</creatorcontrib><creatorcontrib>Loft, Steffen</creatorcontrib><creatorcontrib>Fayomi, Benjamin</creatorcontrib><creatorcontrib>Sanni, Ambaliou</creatorcontrib><creatorcontrib>Vinzents, Peter</creatorcontrib><creatorcontrib>Møller, Peter</creatorcontrib><title>Ultrafine particulate matter and high-level benzene urban air pollution in relation to oxidative DNA damage</title><title>Carcinogenesis (New York)</title><addtitle>Carcinogenesis</addtitle><description>Air pollution, containing high-level of ultrafine particles (UFP) and benzene, is a prominent environmental health problem in many cities of the World. We investigated the level of oxidative DNA damage in mononuclear blood cells (MNBC) by the comet assay as DNA strand breaks (SB) and formamidopyrimidine DNA glycosylase (FPG) sensitive sites in residents from three urban locations in Cotonou, Benin (taxi-moto drivers, subjects living near roads with intense traffic and suburban residents) and rural residents. Exposure was characterized by urinary excretion of S-phenylmercapturic acid (S-PMA), a biomarker of benzene exposure, and by ambient UFP. There were clear stepwise gradients with respect to ambient UFP, S-PMA excretion and oxidative DNA damage with rural subjects < suburban subjects < residents living near highly trafficed roads<taxi-moto drivers. Polymorphisms in glutathione peroxidase (GPX), NAD(P)H:quinone oxidoreductase 1 (NQO1) and glutathione S-transferase (GST) genes were assessed for effect modification. Subjects with GSTT1 null genotype had lower urinary S-PMA excretion than subjects carrying the plus genotype. Urinary S-PMA excretion correlated with SB (R = 0.17) and FPG sites (R = 0.25) in MNBC. The correlation between S-PMA and SB was strongest in subjects with NQO1*1/*2 and *2/*2 genotypes (R = 0.37), and between S-PMA and FPG sensitive sites in subjects with the GSTP1*B/*B genotype (R = 0.39). In conclusion, this study shows that urban air with high levels of benzene and UFP is associated with elevated levels of SB and FPG sites in MNBC, and that NQO1 and GST genes may modulate the effect.</description><subject>8 dihydro-2′-deoxyguanosine</subject><subject>8-oxo-7</subject><subject>8-oxodG</subject><subject>Air</subject><subject>Air Pollutants - analysis</subject><subject>Air Pollutants - toxicity</subject><subject>Benzene - analysis</subject><subject>Benzene - toxicity</subject><subject>Biological and medical sciences</subject><subject>Biomarkers - analysis</subject><subject>Carcinogenesis, carcinogens and anticarcinogens</subject><subject>DNA Damage</subject><subject>Environmental pollutants toxicology</subject><subject>formamidopyrimidine DNA glycosylase</subject><subject>FPG</subject><subject>general linear model</subject><subject>GLM</subject><subject>glutathione peroxidase</subject><subject>glutathione S-transferase</subject><subject>GPX</subject><subject>GST</subject><subject>Humans</subject><subject>least statistical difference</subject><subject>LSD</subject><subject>Male</subject><subject>Medical sciences</subject><subject>MNBC</subject><subject>mononuclear blood cells</subject><subject>NAD(P)H:quinone oxidoreductase 1</subject><subject>NQO1</subject><subject>Oxidative Stress</subject><subject>PAH</subject><subject>Particle Size</subject><subject>particulate matter</subject><subject>polyaromatic hydrocarbons</subject><subject>reactive oxygen species</subject><subject>ROS</subject><subject>S-phenylmercapturic acid</subject><subject>S-PMA</subject><subject>strand breaks</subject><subject>Toxicology</subject><subject>Tumors</subject><subject>UFP</subject><subject>ultrafine particles</subject><subject>Urban Health</subject><issn>0143-3334</issn><issn>1460-2180</issn><issn>1460-2180</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpd0c9vFCEUB_CJ0di1evRqiInexsIwMMOxqa41aWpirDFeyBt4s0vLMCswTfWvl2Y3beKBAOHD48e3ql4z-oFRxU8MROPCybDZcsGfVCvWSlo3rKdPqxVlLa855-1R9SKla0qZ5EI9r46YEIrRXq2qmyufI4wuINlBzM4sHjKSCXLGSCBYsnWbbe3xFj0ZMPzFIpc4QCDgItnN3i_ZzYG4QCKWvffjPJP5ztkyuUXy8fKUWJhggy-rZyP4hK8O_XF1tf70_ey8vvj6-cvZ6UVtWiFzjWAoH0GBaYempVYo6IyUDVprpBXGSDVQip0tzxtbaVrTW2xGIS3rxagGfly939fdxfn3ginrySWD3kPAeUmadX2jGkULfPsfvJ6XGMrddMMUp6K0guo9MnFOKeKod9FNEP9oRvV9BHofgd5HUPybQ9FlmNA-6sOfF_DuACAZ8GOEYFx6dFIo1ZVKDwe7lPHuYR3ijZYd74Q-__lL__gmWcvWl3rN_wE9zaFq</recordid><startdate>20050301</startdate><enddate>20050301</enddate><creator>Avogbe, Patrice H.</creator><creator>Ayi-Fanou, Lucie</creator><creator>Autrup, Herman</creator><creator>Loft, Steffen</creator><creator>Fayomi, Benjamin</creator><creator>Sanni, Ambaliou</creator><creator>Vinzents, Peter</creator><creator>Møller, Peter</creator><general>Oxford University Press</general><general>Oxford Publishing Limited (England)</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>7TM</scope><scope>7TO</scope><scope>7U7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>K9.</scope><scope>P64</scope><scope>RC3</scope></search><sort><creationdate>20050301</creationdate><title>Ultrafine particulate matter and high-level benzene urban air pollution in relation to oxidative DNA damage</title><author>Avogbe, Patrice H. ; Ayi-Fanou, Lucie ; Autrup, Herman ; Loft, Steffen ; Fayomi, Benjamin ; Sanni, Ambaliou ; Vinzents, Peter ; Møller, Peter</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c456t-eac03fa9ac4b240d59a7c662eddc6d5cc69b00e7d014f46c4c8de2f56d185f9b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2005</creationdate><topic>8 dihydro-2′-deoxyguanosine</topic><topic>8-oxo-7</topic><topic>8-oxodG</topic><topic>Air</topic><topic>Air Pollutants - analysis</topic><topic>Air Pollutants - toxicity</topic><topic>Benzene - analysis</topic><topic>Benzene - toxicity</topic><topic>Biological and medical sciences</topic><topic>Biomarkers - analysis</topic><topic>Carcinogenesis, carcinogens and anticarcinogens</topic><topic>DNA Damage</topic><topic>Environmental pollutants toxicology</topic><topic>formamidopyrimidine DNA glycosylase</topic><topic>FPG</topic><topic>general linear model</topic><topic>GLM</topic><topic>glutathione peroxidase</topic><topic>glutathione S-transferase</topic><topic>GPX</topic><topic>GST</topic><topic>Humans</topic><topic>least statistical difference</topic><topic>LSD</topic><topic>Male</topic><topic>Medical sciences</topic><topic>MNBC</topic><topic>mononuclear blood cells</topic><topic>NAD(P)H:quinone oxidoreductase 1</topic><topic>NQO1</topic><topic>Oxidative Stress</topic><topic>PAH</topic><topic>Particle Size</topic><topic>particulate matter</topic><topic>polyaromatic hydrocarbons</topic><topic>reactive oxygen species</topic><topic>ROS</topic><topic>S-phenylmercapturic acid</topic><topic>S-PMA</topic><topic>strand breaks</topic><topic>Toxicology</topic><topic>Tumors</topic><topic>UFP</topic><topic>ultrafine particles</topic><topic>Urban Health</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Avogbe, Patrice H.</creatorcontrib><creatorcontrib>Ayi-Fanou, Lucie</creatorcontrib><creatorcontrib>Autrup, Herman</creatorcontrib><creatorcontrib>Loft, Steffen</creatorcontrib><creatorcontrib>Fayomi, Benjamin</creatorcontrib><creatorcontrib>Sanni, Ambaliou</creatorcontrib><creatorcontrib>Vinzents, Peter</creatorcontrib><creatorcontrib>Møller, Peter</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Oncogenes and Growth Factors Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><jtitle>Carcinogenesis (New York)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Avogbe, Patrice H.</au><au>Ayi-Fanou, Lucie</au><au>Autrup, Herman</au><au>Loft, Steffen</au><au>Fayomi, Benjamin</au><au>Sanni, Ambaliou</au><au>Vinzents, Peter</au><au>Møller, Peter</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Ultrafine particulate matter and high-level benzene urban air pollution in relation to oxidative DNA damage</atitle><jtitle>Carcinogenesis (New York)</jtitle><addtitle>Carcinogenesis</addtitle><date>2005-03-01</date><risdate>2005</risdate><volume>26</volume><issue>3</issue><spage>613</spage><epage>620</epage><pages>613-620</pages><issn>0143-3334</issn><issn>1460-2180</issn><eissn>1460-2180</eissn><coden>CRNGDP</coden><abstract>Air pollution, containing high-level of ultrafine particles (UFP) and benzene, is a prominent environmental health problem in many cities of the World. We investigated the level of oxidative DNA damage in mononuclear blood cells (MNBC) by the comet assay as DNA strand breaks (SB) and formamidopyrimidine DNA glycosylase (FPG) sensitive sites in residents from three urban locations in Cotonou, Benin (taxi-moto drivers, subjects living near roads with intense traffic and suburban residents) and rural residents. Exposure was characterized by urinary excretion of S-phenylmercapturic acid (S-PMA), a biomarker of benzene exposure, and by ambient UFP. There were clear stepwise gradients with respect to ambient UFP, S-PMA excretion and oxidative DNA damage with rural subjects < suburban subjects < residents living near highly trafficed roads<taxi-moto drivers. Polymorphisms in glutathione peroxidase (GPX), NAD(P)H:quinone oxidoreductase 1 (NQO1) and glutathione S-transferase (GST) genes were assessed for effect modification. Subjects with GSTT1 null genotype had lower urinary S-PMA excretion than subjects carrying the plus genotype. Urinary S-PMA excretion correlated with SB (R = 0.17) and FPG sites (R = 0.25) in MNBC. The correlation between S-PMA and SB was strongest in subjects with NQO1*1/*2 and *2/*2 genotypes (R = 0.37), and between S-PMA and FPG sensitive sites in subjects with the GSTP1*B/*B genotype (R = 0.39). In conclusion, this study shows that urban air with high levels of benzene and UFP is associated with elevated levels of SB and FPG sites in MNBC, and that NQO1 and GST genes may modulate the effect.</abstract><cop>Oxford</cop><pub>Oxford University Press</pub><pmid>15591089</pmid><doi>10.1093/carcin/bgh353</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record> |
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source | Oxford University Press Journals All Titles (1996-Current); MEDLINE; EZB-FREE-00999 freely available EZB journals; Alma/SFX Local Collection |
subjects | 8 dihydro-2′-deoxyguanosine 8-oxo-7 8-oxodG Air Air Pollutants - analysis Air Pollutants - toxicity Benzene - analysis Benzene - toxicity Biological and medical sciences Biomarkers - analysis Carcinogenesis, carcinogens and anticarcinogens DNA Damage Environmental pollutants toxicology formamidopyrimidine DNA glycosylase FPG general linear model GLM glutathione peroxidase glutathione S-transferase GPX GST Humans least statistical difference LSD Male Medical sciences MNBC mononuclear blood cells NAD(P)H:quinone oxidoreductase 1 NQO1 Oxidative Stress PAH Particle Size particulate matter polyaromatic hydrocarbons reactive oxygen species ROS S-phenylmercapturic acid S-PMA strand breaks Toxicology Tumors UFP ultrafine particles Urban Health |
title | Ultrafine particulate matter and high-level benzene urban air pollution in relation to oxidative DNA damage |
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