Ultrafine particulate matter and high-level benzene urban air pollution in relation to oxidative DNA damage

Air pollution, containing high-level of ultrafine particles (UFP) and benzene, is a prominent environmental health problem in many cities of the World. We investigated the level of oxidative DNA damage in mononuclear blood cells (MNBC) by the comet assay as DNA strand breaks (SB) and formamidopyrimi...

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Veröffentlicht in:Carcinogenesis (New York) 2005-03, Vol.26 (3), p.613-620
Hauptverfasser: Avogbe, Patrice H., Ayi-Fanou, Lucie, Autrup, Herman, Loft, Steffen, Fayomi, Benjamin, Sanni, Ambaliou, Vinzents, Peter, Møller, Peter
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container_end_page 620
container_issue 3
container_start_page 613
container_title Carcinogenesis (New York)
container_volume 26
creator Avogbe, Patrice H.
Ayi-Fanou, Lucie
Autrup, Herman
Loft, Steffen
Fayomi, Benjamin
Sanni, Ambaliou
Vinzents, Peter
Møller, Peter
description Air pollution, containing high-level of ultrafine particles (UFP) and benzene, is a prominent environmental health problem in many cities of the World. We investigated the level of oxidative DNA damage in mononuclear blood cells (MNBC) by the comet assay as DNA strand breaks (SB) and formamidopyrimidine DNA glycosylase (FPG) sensitive sites in residents from three urban locations in Cotonou, Benin (taxi-moto drivers, subjects living near roads with intense traffic and suburban residents) and rural residents. Exposure was characterized by urinary excretion of S-phenylmercapturic acid (S-PMA), a biomarker of benzene exposure, and by ambient UFP. There were clear stepwise gradients with respect to ambient UFP, S-PMA excretion and oxidative DNA damage with rural subjects < suburban subjects < residents living near highly trafficed roads
doi_str_mv 10.1093/carcin/bgh353
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We investigated the level of oxidative DNA damage in mononuclear blood cells (MNBC) by the comet assay as DNA strand breaks (SB) and formamidopyrimidine DNA glycosylase (FPG) sensitive sites in residents from three urban locations in Cotonou, Benin (taxi-moto drivers, subjects living near roads with intense traffic and suburban residents) and rural residents. Exposure was characterized by urinary excretion of S-phenylmercapturic acid (S-PMA), a biomarker of benzene exposure, and by ambient UFP. There were clear stepwise gradients with respect to ambient UFP, S-PMA excretion and oxidative DNA damage with rural subjects &lt; suburban subjects &lt; residents living near highly trafficed roads&lt;taxi-moto drivers. Polymorphisms in glutathione peroxidase (GPX), NAD(P)H:quinone oxidoreductase 1 (NQO1) and glutathione S-transferase (GST) genes were assessed for effect modification. Subjects with GSTT1 null genotype had lower urinary S-PMA excretion than subjects carrying the plus genotype. Urinary S-PMA excretion correlated with SB (R = 0.17) and FPG sites (R = 0.25) in MNBC. The correlation between S-PMA and SB was strongest in subjects with NQO1*1/*2 and *2/*2 genotypes (R = 0.37), and between S-PMA and FPG sensitive sites in subjects with the GSTP1*B/*B genotype (R = 0.39). 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We investigated the level of oxidative DNA damage in mononuclear blood cells (MNBC) by the comet assay as DNA strand breaks (SB) and formamidopyrimidine DNA glycosylase (FPG) sensitive sites in residents from three urban locations in Cotonou, Benin (taxi-moto drivers, subjects living near roads with intense traffic and suburban residents) and rural residents. Exposure was characterized by urinary excretion of S-phenylmercapturic acid (S-PMA), a biomarker of benzene exposure, and by ambient UFP. There were clear stepwise gradients with respect to ambient UFP, S-PMA excretion and oxidative DNA damage with rural subjects &lt; suburban subjects &lt; residents living near highly trafficed roads&lt;taxi-moto drivers. Polymorphisms in glutathione peroxidase (GPX), NAD(P)H:quinone oxidoreductase 1 (NQO1) and glutathione S-transferase (GST) genes were assessed for effect modification. Subjects with GSTT1 null genotype had lower urinary S-PMA excretion than subjects carrying the plus genotype. Urinary S-PMA excretion correlated with SB (R = 0.17) and FPG sites (R = 0.25) in MNBC. The correlation between S-PMA and SB was strongest in subjects with NQO1*1/*2 and *2/*2 genotypes (R = 0.37), and between S-PMA and FPG sensitive sites in subjects with the GSTP1*B/*B genotype (R = 0.39). 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We investigated the level of oxidative DNA damage in mononuclear blood cells (MNBC) by the comet assay as DNA strand breaks (SB) and formamidopyrimidine DNA glycosylase (FPG) sensitive sites in residents from three urban locations in Cotonou, Benin (taxi-moto drivers, subjects living near roads with intense traffic and suburban residents) and rural residents. Exposure was characterized by urinary excretion of S-phenylmercapturic acid (S-PMA), a biomarker of benzene exposure, and by ambient UFP. There were clear stepwise gradients with respect to ambient UFP, S-PMA excretion and oxidative DNA damage with rural subjects &lt; suburban subjects &lt; residents living near highly trafficed roads&lt;taxi-moto drivers. Polymorphisms in glutathione peroxidase (GPX), NAD(P)H:quinone oxidoreductase 1 (NQO1) and glutathione S-transferase (GST) genes were assessed for effect modification. Subjects with GSTT1 null genotype had lower urinary S-PMA excretion than subjects carrying the plus genotype. Urinary S-PMA excretion correlated with SB (R = 0.17) and FPG sites (R = 0.25) in MNBC. The correlation between S-PMA and SB was strongest in subjects with NQO1*1/*2 and *2/*2 genotypes (R = 0.37), and between S-PMA and FPG sensitive sites in subjects with the GSTP1*B/*B genotype (R = 0.39). In conclusion, this study shows that urban air with high levels of benzene and UFP is associated with elevated levels of SB and FPG sites in MNBC, and that NQO1 and GST genes may modulate the effect.</abstract><cop>Oxford</cop><pub>Oxford University Press</pub><pmid>15591089</pmid><doi>10.1093/carcin/bgh353</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record>
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source Oxford University Press Journals All Titles (1996-Current); MEDLINE; EZB-FREE-00999 freely available EZB journals; Alma/SFX Local Collection
subjects 8 dihydro-2′-deoxyguanosine
8-oxo-7
8-oxodG
Air
Air Pollutants - analysis
Air Pollutants - toxicity
Benzene - analysis
Benzene - toxicity
Biological and medical sciences
Biomarkers - analysis
Carcinogenesis, carcinogens and anticarcinogens
DNA Damage
Environmental pollutants toxicology
formamidopyrimidine DNA glycosylase
FPG
general linear model
GLM
glutathione peroxidase
glutathione S-transferase
GPX
GST
Humans
least statistical difference
LSD
Male
Medical sciences
MNBC
mononuclear blood cells
NAD(P)H:quinone oxidoreductase 1
NQO1
Oxidative Stress
PAH
Particle Size
particulate matter
polyaromatic hydrocarbons
reactive oxygen species
ROS
S-phenylmercapturic acid
S-PMA
strand breaks
Toxicology
Tumors
UFP
ultrafine particles
Urban Health
title Ultrafine particulate matter and high-level benzene urban air pollution in relation to oxidative DNA damage
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