Identification of Chinese cabbage genes up-regulated by prolonged cold by using microarray analysis
Vernalization is induced by prolonged cold and accelerates flowering. To monitor the genome-wide transcriptome change that identifies the genes that are differently up-regulated depending on the duration of cold exposure, we fabricated a Chinese cabbage cDNA microarray and used it to identify genes...
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Veröffentlicht in: | Plant science (Limerick) 2005-04, Vol.168 (4), p.959-966 |
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Format: | Artikel |
Sprache: | eng |
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Zusammenfassung: | Vernalization is induced by prolonged cold and accelerates flowering. To monitor the genome-wide transcriptome change that identifies the genes that are differently up-regulated depending on the duration of cold exposure, we fabricated a Chinese cabbage cDNA microarray and used it to identify genes that are up-regulated by short-term (1-week) and long-term (4-week) cold. The overall expression patterns between 1- and 4-week cold treated seedlings were very similar but some genes in the cell growth/division/DNA synthesis functional category were regulated differently. Prolonged cold specifically up-regulated several
heat shock proteins (
HSPs), and
small HSPs were more significant. Among the up-regulated
sHSPs, we chose the
small HSP 17.6-II and confirmed its up-regulation in response to long- but not short-term cold exposure by Northern analysis. And its constitutive overexpression moderately accelerated flowering. |
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ISSN: | 0168-9452 1873-2259 |
DOI: | 10.1016/j.plantsci.2004.11.011 |