Hydrolysis of starch particles using immobilized barley α-amylase

Hydrolysis of starch particles using immobilized barley α-amylase

Barley α-amylase has been immobilized on silica particles with diameters between 0.5 and 10 μm using a covalent binding method. Immobilization procedures were adjusted to optimize enzyme activity. The effects of product inhibition, thermal stability and operational stability have been determined. Th...

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Veröffentlicht in:Biochemical engineering journal 2003, Vol.13 (1), p.53-62
Hauptverfasser: Lim, Leng Hong, Macdonald, Douglas G, Hill, Gordon A
Format: Artikel
Sprache:eng
Schlagworte:
Biological and medical sciences
Biotechnology
Degradation
Food processing
Fundamental and applied biological sciences. Psychology
Immobilized enzyme
Industrial applications and implications. Economical aspects
Inhibition
Stability
Starch granules
Triticum aestivum
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Zusammenfassung:Barley α-amylase has been immobilized on silica particles with diameters between 0.5 and 10 μm using a covalent binding method. Immobilization procedures were adjusted to optimize enzyme activity. The effects of product inhibition, thermal stability and operational stability have been determined. The feasibility of using the immobilized enzyme to hydrolyze wheat starch particles at temperatures below the gelatinization temperature (
ISSN:1369-703X
1873-295X
DOI:10.1016/S1369-703X(02)00101-8