Quantitative analysis of agnor counts of buccal mucosal cells of chewers and non chewers of gutkha: A comparative cytologic study
Aims and Objectives: The present study was taken up to evaluate the AgNOR counts in the buccal mucosa cells of gutkha chewers and compare that with the sex-matched controls. Materials and Methods: In all, 100 gutkha chewers and 50 sex-matched non-chewers (controls) were chosen. None of the patients...
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Veröffentlicht in: | Journal of cancer research and therapeutics 2016-01, Vol.12 (1), p.228-231 |
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Sprache: | eng |
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Zusammenfassung: | Aims and Objectives: The present study was taken up to evaluate the AgNOR counts in the buccal mucosa cells of gutkha chewers and compare that with the sex-matched controls.
Materials and Methods: In all, 100 gutkha chewers and 50 sex-matched non-chewers (controls) were chosen. None of the patients in both groups had any clinical oral lesions or systemic diseases. After rinsing with 0.9% sodium chloride, cytologic smears were prepared and stained using the AgNOR method and observed in immersion oil at 1000 × magnification. Finally, 50 cells were selected at random; AgNOR dots were counted and their mean was recorded. The student t-test was used for analysis of data.
Results: Comparison between mean AgNOR counts of gutkha chewers (2.68 ± 0.23) and non-chewers (2.01 ± 0.14) was found to be statistically significant.
Conclusion: Cytology associated with AgNOR staining can effectively detect the early molecular changes within buccal mucosa cells of oral mucosa. |
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ISSN: | 0973-1482 1998-4138 |
DOI: | 10.4103/0973-1482.148713 |