Metabolic responses of CHO cells to limitation of key amino acids

ABSTRACT Chinese hamster ovary (CHO) cells are the predominant host for production of therapeutic glycoproteins. In particular, the glutamine‐synthetase (GS) expression system has been widely used in the biopharmaceutical industry for efficient selection of high‐yielding clones. However, much remain...

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Veröffentlicht in:	Biotechnology and bioengineering 2014-10, Vol.111 (10), p.2095-2106
Hauptverfasser:	Duarte, Tiago M., Carinhas, Nuno, Barreiro, Laura C., Carrondo, Manuel J. T., Alves, Paula M., Teixeira, Ana P.
Format:	Artikel
Sprache:	eng
Schlagworte:	13C-tracer study 1H-NMR analysis Alanine Amino acids Amino Acids - metabolism Ammonia Animals Asparagine - metabolism asparagine modulation Cell Culture Techniques Cells CHO Cells - metabolism Citric Acid Cycle Cricetinae Cricetulus Culture Formates Gas Chromatography-Mass Spectrometry GC-MS analysis Glutamate-Ammonia Ligase - metabolism Glycoproteins GS-CHO cells Labels Magnetic Resonance Spectroscopy Metabolism Metabolites Pyruvates Pyruvic Acid - metabolism Rodents Secretions Serine - metabolism serine modulation Synthesis
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