Quercetin is an effective inhibitor of quorum sensing, biofilm formation and virulence factors in Pseudomonas aeruginosa
Aims The study aimed to perform a systematic investigation of the effects of quercetin on biofilm formation and virulence factors in Pseudomonas aeruginosa. Methods and Results The Ps. aeruginosa strain PAO1 was selected as the test strain. The results indicated that quercetin did not impact the gro...
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| Veröffentlicht in: | Journal of applied microbiology 2016-04, Vol.120 (4), p.966-974 |
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| container_title | Journal of applied microbiology |
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| creator | Ouyang, J. Sun, F. Feng, W. Sun, Y. Qiu, X. Xiong, L. Liu, Y. Chen, Y. |
| description | Aims
The study aimed to perform a systematic investigation of the effects of quercetin on biofilm formation and virulence factors in Pseudomonas aeruginosa.
Methods and Results
The Ps. aeruginosa strain PAO1 was selected as the test strain. The results indicated that quercetin did not impact the growth of PAO1 as determined by MIC and growth curve analysis. However, this compound significantly inhibited (P |
| doi_str_mv | 10.1111/jam.13073 |
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| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_1780528252</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>4001276031</sourcerecordid><originalsourceid>FETCH-LOGICAL-c4873-b1a27dbffdd0702ff8c80a5ffbf3ba61aa0a9813aed1334f786c9801f17045d53</originalsourceid><addsrcrecordid>eNqN0UtrFjEUBuAgir3owj8gATcKTpvL5NJlKVqVigq6HjKZc2o-ZpI2mVT7783Xr7oQBLNJFs95w-El5BlnR7yd441bjrhkRj4g-1xq1QltxMO7d98pZsQeOShlw1hDSj8me0JbZnut9snPLxWyhzVEGgp1kQIi-DXcAA3xexjDmjJNSK9rynWhBWIJ8fI1HUPCMC8UU17cGlJssxO9CbnOED1QdL5NlhZCPxeoU1pSdO0DyPUyxFTcE_II3Vzg6f19SL69ffP17F138en8_dnpRed7a2Q3cifMNCJOEzNMIFpvmVOII8rRae4ccyeWSwcTl7JHY7U_sYwjN6xXk5KH5OUu9yqn6wplHZZQPMyzi5BqGbixTAkrlPgPagwzWugtffEX3aSaY1tkq7RgUnDe1Kud8jmVkgGHqxwWl28HzoZtc0Nrbrhrrtnn94l1XGD6I39X1cDxDvwIM9z-O2n4cPpxF_kLHU-jgA</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1776203211</pqid></control><display><type>article</type><title>Quercetin is an effective inhibitor of quorum sensing, biofilm formation and virulence factors in Pseudomonas aeruginosa</title><source>Oxford University Press Journals All Titles (1996-Current)</source><source>MEDLINE</source><source>Wiley Online Library Journals Frontfile Complete</source><creator>Ouyang, J. ; Sun, F. ; Feng, W. ; Sun, Y. ; Qiu, X. ; Xiong, L. ; Liu, Y. ; Chen, Y.</creator><creatorcontrib>Ouyang, J. ; Sun, F. ; Feng, W. ; Sun, Y. ; Qiu, X. ; Xiong, L. ; Liu, Y. ; Chen, Y.</creatorcontrib><description>Aims
The study aimed to perform a systematic investigation of the effects of quercetin on biofilm formation and virulence factors in Pseudomonas aeruginosa.
Methods and Results
The Ps. aeruginosa strain PAO1 was selected as the test strain. The results indicated that quercetin did not impact the growth of PAO1 as determined by MIC and growth curve analysis. However, this compound significantly inhibited (P < 0·05) biofilm formation and production of virulence factors including pyocyanin, protease and elastase at a lower concentration than those for most previously reported plant extracts and substances. Considering the central role of quorum sensing (QS) in the regulation of biofilm and virulence factor, we further detected the transcriptional changes associated with QS and found that the expression levels of lasI, lasR, rhlI and rhlR were significantly reduced (P < 0·05) by 34, 68, 57 and 50%, respectively, in response to 16 μg ml−1 quercetin.
Conclusions
This study indicated that quercetin is an effective inhibitor of biofilm formation and virulence factors in Ps. aeruginosa.
Significance and Impact of the Study
This is the first study to demonstrate that quercetin is an effective inhibitor of QS, biofilm formation and virulence factors in Ps. aeruginosa. Furthermore, quercetin might have potential in fighting biofilm‐related infections.</description><identifier>ISSN: 1364-5072</identifier><identifier>EISSN: 1365-2672</identifier><identifier>DOI: 10.1111/jam.13073</identifier><identifier>PMID: 26808465</identifier><identifier>CODEN: JAMIFK</identifier><language>eng</language><publisher>England: Oxford University Press</publisher><subject>Bacteriology ; biofilm ; Biofilms ; Biofilms - drug effects ; Biofilms - growth & development ; Microbiology ; Pseudomonas aeruginosa ; Pseudomonas aeruginosa - drug effects ; Pseudomonas aeruginosa - physiology ; quercetin ; Quercetin - pharmacology ; quorum sensing ; Quorum Sensing - drug effects ; Virology ; virulence factors ; Virulence Factors - analysis ; Virulence Factors - biosynthesis</subject><ispartof>Journal of applied microbiology, 2016-04, Vol.120 (4), p.966-974</ispartof><rights>2016 The Society for Applied Microbiology</rights><rights>2016 The Society for Applied Microbiology.</rights><rights>Copyright © 2016 The Society for Applied Microbiology</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4873-b1a27dbffdd0702ff8c80a5ffbf3ba61aa0a9813aed1334f786c9801f17045d53</citedby><cites>FETCH-LOGICAL-c4873-b1a27dbffdd0702ff8c80a5ffbf3ba61aa0a9813aed1334f786c9801f17045d53</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fjam.13073$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fjam.13073$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27903,27904,45553,45554</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/26808465$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ouyang, J.</creatorcontrib><creatorcontrib>Sun, F.</creatorcontrib><creatorcontrib>Feng, W.</creatorcontrib><creatorcontrib>Sun, Y.</creatorcontrib><creatorcontrib>Qiu, X.</creatorcontrib><creatorcontrib>Xiong, L.</creatorcontrib><creatorcontrib>Liu, Y.</creatorcontrib><creatorcontrib>Chen, Y.</creatorcontrib><title>Quercetin is an effective inhibitor of quorum sensing, biofilm formation and virulence factors in Pseudomonas aeruginosa</title><title>Journal of applied microbiology</title><addtitle>J Appl Microbiol</addtitle><description>Aims
The study aimed to perform a systematic investigation of the effects of quercetin on biofilm formation and virulence factors in Pseudomonas aeruginosa.
Methods and Results
The Ps. aeruginosa strain PAO1 was selected as the test strain. The results indicated that quercetin did not impact the growth of PAO1 as determined by MIC and growth curve analysis. However, this compound significantly inhibited (P < 0·05) biofilm formation and production of virulence factors including pyocyanin, protease and elastase at a lower concentration than those for most previously reported plant extracts and substances. Considering the central role of quorum sensing (QS) in the regulation of biofilm and virulence factor, we further detected the transcriptional changes associated with QS and found that the expression levels of lasI, lasR, rhlI and rhlR were significantly reduced (P < 0·05) by 34, 68, 57 and 50%, respectively, in response to 16 μg ml−1 quercetin.
Conclusions
This study indicated that quercetin is an effective inhibitor of biofilm formation and virulence factors in Ps. aeruginosa.
Significance and Impact of the Study
This is the first study to demonstrate that quercetin is an effective inhibitor of QS, biofilm formation and virulence factors in Ps. aeruginosa. Furthermore, quercetin might have potential in fighting biofilm‐related infections.</description><subject>Bacteriology</subject><subject>biofilm</subject><subject>Biofilms</subject><subject>Biofilms - drug effects</subject><subject>Biofilms - growth & development</subject><subject>Microbiology</subject><subject>Pseudomonas aeruginosa</subject><subject>Pseudomonas aeruginosa - drug effects</subject><subject>Pseudomonas aeruginosa - physiology</subject><subject>quercetin</subject><subject>Quercetin - pharmacology</subject><subject>quorum sensing</subject><subject>Quorum Sensing - drug effects</subject><subject>Virology</subject><subject>virulence factors</subject><subject>Virulence Factors - analysis</subject><subject>Virulence Factors - biosynthesis</subject><issn>1364-5072</issn><issn>1365-2672</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqN0UtrFjEUBuAgir3owj8gATcKTpvL5NJlKVqVigq6HjKZc2o-ZpI2mVT7783Xr7oQBLNJFs95w-El5BlnR7yd441bjrhkRj4g-1xq1QltxMO7d98pZsQeOShlw1hDSj8me0JbZnut9snPLxWyhzVEGgp1kQIi-DXcAA3xexjDmjJNSK9rynWhBWIJ8fI1HUPCMC8UU17cGlJssxO9CbnOED1QdL5NlhZCPxeoU1pSdO0DyPUyxFTcE_II3Vzg6f19SL69ffP17F138en8_dnpRed7a2Q3cifMNCJOEzNMIFpvmVOII8rRae4ccyeWSwcTl7JHY7U_sYwjN6xXk5KH5OUu9yqn6wplHZZQPMyzi5BqGbixTAkrlPgPagwzWugtffEX3aSaY1tkq7RgUnDe1Kud8jmVkgGHqxwWl28HzoZtc0Nrbrhrrtnn94l1XGD6I39X1cDxDvwIM9z-O2n4cPpxF_kLHU-jgA</recordid><startdate>201604</startdate><enddate>201604</enddate><creator>Ouyang, J.</creator><creator>Sun, F.</creator><creator>Feng, W.</creator><creator>Sun, Y.</creator><creator>Qiu, X.</creator><creator>Xiong, L.</creator><creator>Liu, Y.</creator><creator>Chen, Y.</creator><general>Oxford University Press</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7QO</scope><scope>7T7</scope><scope>7TM</scope><scope>7U7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><scope>7ST</scope><scope>SOI</scope></search><sort><creationdate>201604</creationdate><title>Quercetin is an effective inhibitor of quorum sensing, biofilm formation and virulence factors in Pseudomonas aeruginosa</title><author>Ouyang, J. ; Sun, F. ; Feng, W. ; Sun, Y. ; Qiu, X. ; Xiong, L. ; Liu, Y. ; Chen, Y.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4873-b1a27dbffdd0702ff8c80a5ffbf3ba61aa0a9813aed1334f786c9801f17045d53</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>Bacteriology</topic><topic>biofilm</topic><topic>Biofilms</topic><topic>Biofilms - drug effects</topic><topic>Biofilms - growth & development</topic><topic>Microbiology</topic><topic>Pseudomonas aeruginosa</topic><topic>Pseudomonas aeruginosa - drug effects</topic><topic>Pseudomonas aeruginosa - physiology</topic><topic>quercetin</topic><topic>Quercetin - pharmacology</topic><topic>quorum sensing</topic><topic>Quorum Sensing - drug effects</topic><topic>Virology</topic><topic>virulence factors</topic><topic>Virulence Factors - analysis</topic><topic>Virulence Factors - biosynthesis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ouyang, J.</creatorcontrib><creatorcontrib>Sun, F.</creatorcontrib><creatorcontrib>Feng, W.</creatorcontrib><creatorcontrib>Sun, Y.</creatorcontrib><creatorcontrib>Qiu, X.</creatorcontrib><creatorcontrib>Xiong, L.</creatorcontrib><creatorcontrib>Liu, Y.</creatorcontrib><creatorcontrib>Chen, Y.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Nucleic Acids Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>Environment Abstracts</collection><collection>Environment Abstracts</collection><jtitle>Journal of applied microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ouyang, J.</au><au>Sun, F.</au><au>Feng, W.</au><au>Sun, Y.</au><au>Qiu, X.</au><au>Xiong, L.</au><au>Liu, Y.</au><au>Chen, Y.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Quercetin is an effective inhibitor of quorum sensing, biofilm formation and virulence factors in Pseudomonas aeruginosa</atitle><jtitle>Journal of applied microbiology</jtitle><addtitle>J Appl Microbiol</addtitle><date>2016-04</date><risdate>2016</risdate><volume>120</volume><issue>4</issue><spage>966</spage><epage>974</epage><pages>966-974</pages><issn>1364-5072</issn><eissn>1365-2672</eissn><coden>JAMIFK</coden><abstract>Aims
The study aimed to perform a systematic investigation of the effects of quercetin on biofilm formation and virulence factors in Pseudomonas aeruginosa.
Methods and Results
The Ps. aeruginosa strain PAO1 was selected as the test strain. The results indicated that quercetin did not impact the growth of PAO1 as determined by MIC and growth curve analysis. However, this compound significantly inhibited (P < 0·05) biofilm formation and production of virulence factors including pyocyanin, protease and elastase at a lower concentration than those for most previously reported plant extracts and substances. Considering the central role of quorum sensing (QS) in the regulation of biofilm and virulence factor, we further detected the transcriptional changes associated with QS and found that the expression levels of lasI, lasR, rhlI and rhlR were significantly reduced (P < 0·05) by 34, 68, 57 and 50%, respectively, in response to 16 μg ml−1 quercetin.
Conclusions
This study indicated that quercetin is an effective inhibitor of biofilm formation and virulence factors in Ps. aeruginosa.
Significance and Impact of the Study
This is the first study to demonstrate that quercetin is an effective inhibitor of QS, biofilm formation and virulence factors in Ps. aeruginosa. Furthermore, quercetin might have potential in fighting biofilm‐related infections.</abstract><cop>England</cop><pub>Oxford University Press</pub><pmid>26808465</pmid><doi>10.1111/jam.13073</doi><tpages>9</tpages><oa>free_for_read</oa></addata></record> |
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| source | Oxford University Press Journals All Titles (1996-Current); MEDLINE; Wiley Online Library Journals Frontfile Complete |
| subjects | Bacteriology biofilm Biofilms Biofilms - drug effects Biofilms - growth & development Microbiology Pseudomonas aeruginosa Pseudomonas aeruginosa - drug effects Pseudomonas aeruginosa - physiology quercetin Quercetin - pharmacology quorum sensing Quorum Sensing - drug effects Virology virulence factors Virulence Factors - analysis Virulence Factors - biosynthesis |
| title | Quercetin is an effective inhibitor of quorum sensing, biofilm formation and virulence factors in Pseudomonas aeruginosa |
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