Domain antibody downstream process optimization: High-throughput strategy and analytical methods

Domain antibody downstream process optimization: High-throughput strategy and analytical methods

Application of scale‐down high‐throughput screening has become integral for process development of antibody therapeutic products. In this work, methods are described for using high‐throughput techniques to develop a multicolumn chromatography purification protocol for a small domain antibody with ve...

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Veröffentlicht in:Engineering in life sciences 2016-03, Vol.16 (2), p.133-142
Hauptverfasser: Welsh, John P., Rauscher, Michael A., Bao, Haiying, Meissner, Sandra, Han, InKwan, Linden, Thomas O., Pollard, Jennifer M.
Format: Artikel
Sprache:eng
Schlagworte:
Chromatography
Domain antibody
High-throughput process development
Miniature columns
Resin slurry plates
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Zusammenfassung:Application of scale‐down high‐throughput screening has become integral for process development of antibody therapeutic products. In this work, methods are described for using high‐throughput techniques to develop a multicolumn chromatography purification protocol for a small domain antibody with very limited material (
ISSN:1618-0240
1618-2863
DOI:10.1002/elsc.201400255