Structure of Ulvan Isolated from the Edible Green Seaweed, Ulva pertusa

Ulvan, rhamnan sulfate, was extracted from the edible green seaweed, Ana-aosa (Ulva pertusa), which is grown on the coast of the Okinawa Islands. The yield of ulvan was 8.5% (W/W), and the total carbohydrates, uronic acid and sulfuric acid and ash contents were 67.3%, 23.8%, 19.7% and 22.6%, respectively. L-Rhamnose, D-xylose and D-glucose residues were identified by liquid chromatography, and their molar ratio was 4.0:0.1:0.3. D-Glucuronic and L-idulonic acid residues were also identified in molar ratio of 1.0:0.2. The NMR ( super( 13)C and super( 1)H) and methylation analysis revealed terminal beta -D-glucruonic acid, terminal alpha -L-idulonic acid, 1,3-linked alpha -L-rhamnose, 1,4-linked alpha -L-rhamnose, 1,2,4-linked alpha -L-rhamnose, 1,3,4-linked alpha -L-rhamnose, 1,2,3,4-linked alpha -L-rhamnose and 1,3,4-linked beta -D-xylose. The sulfate groups were attached at the C-2 and C-3 positions of the 1,4-linked alpha -L-rhamnose as well as C-3 of the 1,4-linked beta -D-xylose residues. The chemical structure of the ulvan from Ulva pertusa was determined.