Expression of a CaMV::sak-gusA-mgfp gene in tobacco plants

Expression of a CaMV::sak-gusA-mgfp gene in tobacco plants

A gene encoding staphylokinase from Staphylococcus aureus was cloned into the plant transformation binary vector pCAMBIA1303. The presence of a CaMV::sak-gusA-mgfp gene in Agrobacterium was confirmed by polymerase chain reaction PCR. Tobacco seedlings were used as explants for Agrobacterium tumefaci...

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Veröffentlicht in:World journal of microbiology & biotechnology 2004, Vol.20 (3), p.333-335
Hauptverfasser: DOBROWOLSKA, Anna, KONONOWICZ, Andrzej K
Format: Artikel
Sprache:eng
Schlagworte:
Agrobacterium tumefaciens
Bacteria
Biological and medical sciences
Biotechnology
Cauliflower mosaic virus
Fundamental and applied biological sciences. Psychology
Nicotiana tabacum
Seedlings
Staphylococcus aureus
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container_title World journal of microbiology & biotechnology
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creator DOBROWOLSKA, Anna
KONONOWICZ, Andrzej K
description A gene encoding staphylokinase from Staphylococcus aureus was cloned into the plant transformation binary vector pCAMBIA1303. The presence of a CaMV::sak-gusA-mgfp gene in Agrobacterium was confirmed by polymerase chain reaction PCR. Tobacco seedlings were used as explants for Agrobacterium tumefaciens-mediated transformation with the pCAMBIA1303sak vector carrying the fusion gene construct CaMV::sak-gusA-mgfp and the expression of the fusion gene was identified in Nicotiana tabacum plants by β-glucuronidas assay.[PUBLICATION ABSTRACT]
doi_str_mv 10.1023/B:WIBI.0000023910.95960.94
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source springer (창간호~2014)
subjects Agrobacterium tumefaciens
Bacteria
Biological and medical sciences
Biotechnology
Cauliflower mosaic virus
Fundamental and applied biological sciences. Psychology
Nicotiana tabacum
Seedlings
Staphylococcus aureus
title Expression of a CaMV::sak-gusA-mgfp gene in tobacco plants
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