Expression of a CaMV::sak-gusA-mgfp gene in tobacco plants

A gene encoding staphylokinase from Staphylococcus aureus was cloned into the plant transformation binary vector pCAMBIA1303. The presence of a CaMV::sak-gusA-mgfp gene in Agrobacterium was confirmed by polymerase chain reaction PCR. Tobacco seedlings were used as explants for Agrobacterium tumefaci...

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Veröffentlicht in:	World journal of microbiology & biotechnology 2004, Vol.20 (3), p.333-335
Hauptverfasser:	DOBROWOLSKA, Anna, KONONOWICZ, Andrzej K
Format:	Artikel
Sprache:	eng
Schlagworte:	Agrobacterium tumefaciens Bacteria Biological and medical sciences Biotechnology Cauliflower mosaic virus Fundamental and applied biological sciences. Psychology Nicotiana tabacum Seedlings Staphylococcus aureus
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Zusammenfassung:	A gene encoding staphylokinase from Staphylococcus aureus was cloned into the plant transformation binary vector pCAMBIA1303. The presence of a CaMV::sak-gusA-mgfp gene in Agrobacterium was confirmed by polymerase chain reaction PCR. Tobacco seedlings were used as explants for Agrobacterium tumefaciens-mediated transformation with the pCAMBIA1303sak vector carrying the fusion gene construct CaMV::sak-gusA-mgfp and the expression of the fusion gene was identified in Nicotiana tabacum plants by β-glucuronidas assay.[PUBLICATION ABSTRACT]
ISSN:	0959-3993 1573-0972
DOI:	10.1023/B:WIBI.0000023910.95960.94