New expanded bed adsorbents for the recovery of DNA

New expanded bed adsorbents for the recovery of DNA

A 20-40 μm pellicular high density (3.7 g cm^sup -3^) expanded bed material has been designed for the capture of DNA and other large macromolecules. Anion exchangers fashioned out of these supports exhibited dramatically enhanced DNA binding capacities over commercial anion exchange adsorbents (6 mg...

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Veröffentlicht in:Biotechnology letters 2000-12, Vol.22 (24), p.1929-1933
Hauptverfasser: THEODOSSIOU, Irini, OLANDER, Morten A, SØNDERGAARD, Michael, THOMAS, Owen R. T
Format: Artikel
Sprache:eng
Schlagworte:
Adsorbents
Anion exchange
Biological and medical sciences
Biotechnology
Deoxyribonucleic acid
Diverse techniques
DNA
Fundamental and applied biological sciences. Psychology
Molecular and cellular biology
polyethyleneimine
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Zusammenfassung:A 20-40 μm pellicular high density (3.7 g cm^sup -3^) expanded bed material has been designed for the capture of DNA and other large macromolecules. Anion exchangers fashioned out of these supports exhibited dramatically enhanced DNA binding capacities over commercial anion exchange adsorbents (6 mg ml^sup -1^, c.f. 50 μg ml^sup -1^ at 10% breakthrough), due to a combination of small particle and 'fuzzy' surface architecture created through the coupling of polyethylene imine chains.[PUBLICATION ABSTRACT]
ISSN:0141-5492
1573-6776
DOI:10.1023/A:1026708603888