Staphylococcus epidermidis Biofilm Formation and Structural Organization on Different Types of Intraocular Lenses under in vitro Flow Conditions
Aim: To compare the adherence and structural organization of Staphylococcus epidermidis biofilm on intraocular lenses (IOLs). Methods: IOLs made of 3 different biomaterials [polymethyl methacrylate (PMMA), hydrophilic acrylic or hydrophobic acrylic] were incubated into an S. epidermidis bacterial so...
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Veröffentlicht in: | Ophthalmic research 2013-01, Vol.50 (2), p.83-90 |
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Sprache: | eng |
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Zusammenfassung: | Aim: To compare the adherence and structural organization of Staphylococcus epidermidis biofilm on intraocular lenses (IOLs). Methods: IOLs made of 3 different biomaterials [polymethyl methacrylate (PMMA), hydrophilic acrylic or hydrophobic acrylic] were incubated into an S. epidermidis bacterial solution. Scanning electron microscopy was used to count the bound bacteria and to analyze the structural biofilm architecture. Results: After 4-6 h of incubation, adherence was statistically weakest on the hydrophilic acrylic polymer. On the hydrophobic acrylic material, the bacterial cells tended to cover the substratum in a horizontal spread in a continuous monolayer. On the hydrophilic acrylic material or on the PMMA material bacterial cells tended to form only few, small scattered cell clusters. Conclusions: The data suggest that the pattern of S. epidermidis adhesion varies with the IOL biomaterial. Hydrophobic IOLs seem to be more permissive to S. epidermidis adhesion. |
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ISSN: | 0030-3747 1423-0259 |
DOI: | 10.1159/000350550 |