PRODUCTION & CHARACTERIZATION OF ALKALINE PROTEASE FROM LOCALLY ISOLATED ALKALIPHILIC BACILLUS SPECIES

In present study 50 bacterial alkaliphilic Bacillus species were isolated from local habitat. Out of fifty, 5 promising isolates were selected for production of protease enzyme. Horikoshi I media was used in production. Production was carried out at different temperatures, different pH and at different substrate concentrations. Maximum production recorded at 400C and at pH- 10 by isolates 3, 4 and 5 and isolates 1 and 2 produce maximum protease at 400C and at pH - 9. Low substrate concentration were favorable for isolates 1, 2 and 3 while high substrate concentration ( higher than 1,2 and 3) were suitable for 4 and 5 isolates in production of protease.