Evaluation of Ketoprofen Effects on Humoral Immunity and Immune Organs in Mice

The present project was designed to study the effects of ketoprofen on humoral immunity and to evaluate the toxic effects of ketoprofen on immune organs of mice. Effects of ketoprofen on humoral component of immune system were evaluated at two doses 1mg/kg and 5mg/kg by performing haemagglutination assay and mice lethality test. Spleen and thymus were removed from the mice and histopathological examination was performed to observe the toxic effects of ketoprofen on these organs. Ketoprofen treatment exhibited significant (pless than 0.001) decrease in serum anti-SRBCs antibody titer as compared to the control. Mortality ratio was significantly (pless than 0.001) increased in ketoprofen treated mice. There was a significant (p=0.01) decrease in the weight of spleen and thymus in ketoprofen treated groups. Histopathological analysis of spleen of the mice treated with .ketoprofen presented slight increase in the thickness of the trabecular due to odema in spleen. Moreover few medullary changes in the form of mild medullary atrophy occurred in the thymus of mice. These results provide the information that ketoprofen has immunosuppressive effects on humoral immunity therefore it can be used in patients with autoimmune diseases and to prevent allograft rejection.