Transgenic Turfgrasses Expressing Hyperactive Ser599Ala Phytochrome A Mutant Exhibit Abiotic Stress Tolerance

Turfgrasses are environmentally and recreationally valuable plants that are constantly subjected to various forms of stress in their artificial and natural habitats. Previously, it was shown that the transformation of a hyperactive mutant (Serine 599 Alanine, S599A) of oat phytochrome A in zoysia grass (Zoysia japonica) and creeping bentgrass (Agrostis stolonifera L.) resulted in superior quality turfgrass with improved shade tolerance response. We now examined the abiotic stress response of the transgenic turfgrasses expressing the hyperactive mutant S599A-PhyA. The transgenic S599A-PhyA plants subjected to high salinity and heavy metal toxicity stress exhibited higher chlorophyll content, lower hydrogen peroxide level, and higher proline accumulation than the controls. Furthermore, the anti-oxidative activities of four reactive oxygen species scavenging enzymes and the total biomass (above and below-ground) were higher in S599A-PhyA plants than in the controls under both the stress conditions. Moreover, higher photosynthetic efficiency (F ᵥ/F ₘ) of S599A-PhyA plants indicated healthier growth than the controls under stress conditions. Results suggest that the hyperactive mutant of oat phytochrome A confers abiotic stress tolerance in plants, and can be used to efficiently develop abiotic stress tolerant crops in future.