The Interaction of Implant Luting Cements and Oral Bacteria Linked to Peri-Implant Disease: An In Vitro Analysis of Planktonic and Biofilm Growth - A Preliminary Study

Background There is little consensus on the most appropriate cement to use when restoring a cement‐retained, implant‐supported restoration. One consideration should be the interaction of pathogenic oral bacteria with restorative cements. Purpose To determine how oral bacteria associated with peri‐implant disease grow in the presence of implant cements. Materials and Methods Five test cements with varying composition (zinc oxide–eugenol [TBO], eugenol‐free zinc oxide [TBNE], zinc orthophosphate [FL], and two resin cements [PIC and ML]) were used to fabricate specimen disks. The disks were submerged in bacterial suspensions of either Aggregatibacter actinomycetemcomitans, Fusobacterium nucleatum, or Porphyromonas gingivalis. Planktonic bacterial growth within the test media was measured by determining the optical density of the cultures (OD600). Positive controls (media and bacteria without cement disks) and negative controls (media alone) were similarly evaluated. The mean and standard deviations (SD) were calculated for planktonic growth from three separate experiments. ANOVA statistical analysis with post hoc Tukey tests was performed where differences existed (p