Direct organogenesis in Datura metel L. from in vitro and in vivo nodal explants

Direct organogenesis in Datura metel L. from in vitro and in vivo nodal explants

In vitro plant regeneration was achieved in Datura metel from nodal explants collected from both in vitro germinated seedlings and field grown plants (in vivo). The explants were cultured on MS medium with BAP (0.5-3.0 mg/l) and NAA (0.5 mg/l). The nodal explants isolated from in vivo source exhibit...

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Veröffentlicht in:Indian journal of biotechnology 2004-07, Vol.3 (3), p.449-451
Hauptverfasser: Muthukumar, B, Arockiasamy, DI, Natarajan, E
Format: Artikel
Sprache:eng
Schlagworte:
Datura metel
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Zusammenfassung:In vitro plant regeneration was achieved in Datura metel from nodal explants collected from both in vitro germinated seedlings and field grown plants (in vivo). The explants were cultured on MS medium with BAP (0.5-3.0 mg/l) and NAA (0.5 mg/l). The nodal explants isolated from in vivo source exhibited a greater number of healthy multiple shoots than in vitro. BAP at 3 mg/l with NAA at 0.5 mg/l was found to be optimal for regeneration of shootlets.
ISSN:0972-5849