Single Site Discrimination of Cytosine, 5‑Methylcytosine, and 5‑Hydroxymethylcytosine in Target DNA Using Anthracene-Tagged Fluorescent Probes

Single Site Discrimination of Cytosine, 5‑Methylcytosine, and 5‑Hydroxymethylcytosine in Target DNA Using Anthracene-Tagged Fluorescent Probes

The ability to discriminate between epigenetic variants in DNA is a necessary tool if we are to increase our understanding of the roles that they play in various biological processes and medical conditions. Herein, it is demonstrated how a simple two-step fluorescent probe assay can be used to diffe...

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Veröffentlicht in:ACS chemical biology 2016-03, Vol.11 (3), p.717-721
Hauptverfasser: Duprey, Jean-Louis H. A, Bullen, Gemma A, Zhao, Zheng-yun, Bassani, Dario M, Peacock, Anna F. A, Wilkie, John, Tucker, James H. R
Format: Artikel
Sprache:eng
Schlagworte:
5-Methylcytosine - chemistry
Anthracenes - chemistry
Cytosine - analogs & derivatives
Cytosine - chemistry
DNA - chemistry
Fluorescent Dyes - chemistry
Nucleic Acid Conformation
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Zusammenfassung:The ability to discriminate between epigenetic variants in DNA is a necessary tool if we are to increase our understanding of the roles that they play in various biological processes and medical conditions. Herein, it is demonstrated how a simple two-step fluorescent probe assay can be used to differentiate all three major epigenetic variants of cytosine at a single locus site in a target strand of DNA.
ISSN:1554-8929
1554-8937
DOI:10.1021/acschembio.5b00796