A Genetically Encoded Photocaged Amino Acid

We have developed a second orthogonal tRNA/synthetase pair for use in yeast based on the Escherichia coli tRNALeu/leucyl tRNA-synthetase pair. Using a novel genetic selection, we have identified a series of synthetase mutants that selectively charge the amber suppresor tRNA with the C8 amino acid, α...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Veröffentlicht in:	Journal of the American Chemical Society 2004-11, Vol.126 (44), p.14306-14307
Hauptverfasser:	Wu, Ning, Deiters, Alexander, Cropp, T. Ashton, King, David, Schultz, Peter G
Format:	Artikel
Sprache:	eng
Schlagworte:	Base Sequence Biological and medical sciences Biotechnology Caprylates - metabolism Cysteine - analogs & derivatives Cysteine - metabolism Fundamental and applied biological sciences. Psychology Genetic engineering Genetic technics Histidine - genetics Histidine - metabolism Humans Leucine-tRNA Ligase - genetics Leucine-tRNA Ligase - metabolism Methods. Procedures. Technologies Methyltyrosines - genetics Methyltyrosines - metabolism Models, Molecular Molecular Sequence Data Mutagenesis Nucleic Acid Conformation Photochemistry Proteins - genetics RNA, Transfer, Amino Acyl - genetics RNA, Transfer, Amino Acyl - metabolism Saccharomyces cerevisiae Site specific mutagenesis Superoxide Dismutase - genetics
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Beschreibung
Zusammenfassung:	We have developed a second orthogonal tRNA/synthetase pair for use in yeast based on the Escherichia coli tRNALeu/leucyl tRNA-synthetase pair. Using a novel genetic selection, we have identified a series of synthetase mutants that selectively charge the amber suppresor tRNA with the C8 amino acid, α-aminocaprylic acid, and the photocaged amino acid, o-nitrobenzyl cysteine, allowing them to be inserted into proteins in yeast in response to the amber nonsense codon, TAG.
ISSN:	0002-7863 1520-5126
DOI:	10.1021/ja040175z