Design Study of an Atmospheric Pressure Photoionization Interface for GC-MS

Design Study of an Atmospheric Pressure Photoionization Interface for GC-MS

This contribution reports on the development of an atmospheric pressure photoionization (APPI) source interfacing a gas chromatograph (GC) with a bench-top Orbitrap high resolution mass spectrometer (MS). We present efforts on method development aiming at high temperature stability (325°C), constant...

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Veröffentlicht in:Journal of the American Society for Mass Spectrometry 2016-04, Vol.27 (4), p.607-614
Hauptverfasser: Kersten, Hendrik, Kroll, Kai, Haberer, Kirsten, Brockmann, Klaus J., Benter, Thorsten, Peterson, Amelia, Makarov, Alexander
Format: Artikel
Sprache:eng
Schlagworte:
Analytical Chemistry
Atmospheric pressure
Bioinformatics
Biotechnology
Chemistry
Chemistry and Materials Science
Gas chromatography
Mass spectrometry
Organic Chemistry
Photoionization
Proteomics
Research Article
Sensitivity analysis
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Beschreibung
Zusammenfassung:This contribution reports on the development of an atmospheric pressure photoionization (APPI) source interfacing a gas chromatograph (GC) with a bench-top Orbitrap high resolution mass spectrometer (MS). We present efforts on method development aiming at high temperature stability (325°C), constant low impurity levels upon prolonged source operation, and efficient reaction volume irradiation combined with minimum peak broadening. The performance throughout each iterative development step was carefully assessed. The final GC-APPI-MS setup demonstrated femtogram-on-column sensitivity and chromatographic peaks of Gaussian shape with base peak widths
ISSN:1044-0305
1879-1123
DOI:10.1007/s13361-015-1320-x