Isotope effect analyses provide evidence for an altered transition state for RNA 2'-O-transphosphorylation catalyzed by Zn(2+)

Isotope effect analyses provide evidence for an altered transition state for RNA 2'-O-transphosphorylation catalyzed by Zn(2+)

Solvent D2O and (18)O kinetic isotope effects on RNA 2'-O-transphosphorylation catalyzed by Zn(2+) demonstrate an altered transition state relative to specific base catalysis. A recent model from DFT calculations involving inner sphere coordination to the non-bridging and leaving group oxygens...

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Veröffentlicht in:Chemical communications (Cambridge, England) England), 2016-03, Vol.52 (24), p.4462-4465
Hauptverfasser: Zhang, Shuming, Gu, Hong, Chen, Haoyuan, Strong, Emily, Ollie, Edward W, Kellerman, Daniel, Liang, Danni, Miyagi, Masaru, Anderson, Vernon E, Piccirilli, Joseph A, York, Darrin M, Harris, Michael E
Format: Artikel
Sprache:eng
Schlagworte:
Catalysis
Isotopes
Phosphorylation
RNA - chemistry
Zinc - chemistry
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Zusammenfassung:Solvent D2O and (18)O kinetic isotope effects on RNA 2'-O-transphosphorylation catalyzed by Zn(2+) demonstrate an altered transition state relative to specific base catalysis. A recent model from DFT calculations involving inner sphere coordination to the non-bridging and leaving group oxygens is consistent with the data.
ISSN:1364-548X
DOI:10.1039/c5cc10212j