Detection and cloning of expressed sequences linked to a target gene

DNA markers tightly linked to a target gene are essential starting points for positional cloning. We combined "differential display of mRNA" and "bulked segregant analysis" in order to detect and clone ten expressed sequences as markers linked to a virus resistance gene in Phaseolus vulgaris. The combination of these two procedures could be used in lieu of positional cloning, provided polymorphisms detectable by differential display exist in the target gene. Isolation of expressed sequences from specific chromosome regions can also be accomplished by combining these procedures.[PUBLICATION ABSTRACT]