Enhanced paclitaxel production induced by the combination of elicitors in cell suspension cultures of Taxus chinensis

Taxus chinensis suspension cells were cultured in the modified Gamborg's B5 medium. Addition of 50 mg chitosan l^sup -1^, 60 μM methyl jasmonate and 30 μM Ag^sup +^ resulted in the greatest paclitaxel production, at 25 mg l^sup -1^ in the cultures, being almost 40 times higher than that of the...

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Veröffentlicht in:Biotechnology letters 2000-10, Vol.22 (19), p.1561-1564
Hauptverfasser: ZHANG, C. H, MEI, X. G, LIU, L, YU, L. J
Format: Artikel
Sprache:eng
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Zusammenfassung:Taxus chinensis suspension cells were cultured in the modified Gamborg's B5 medium. Addition of 50 mg chitosan l^sup -1^, 60 μM methyl jasmonate and 30 μM Ag^sup +^ resulted in the greatest paclitaxel production, at 25 mg l^sup -1^ in the cultures, being almost 40 times higher than that of the control culture, 10 times higher than that of the culture exposed to Ag^sup +^, 6 times higher than that of the culture elicited by chitosan and almost double that of the culture elicited by methyl jasmonate.[PUBLICATION ABSTRACT]
ISSN:0141-5492
1573-6776
DOI:10.1023/A:1005684901329