Rice Stomatal Closure Requires Guard Cell Plasma Membrane ATP-Binding Cassette Transporter RCN1/OsABCG5

Water stress is one of the major environmental stresses that affect agricultural production worldwide. Water loss from plants occurs primarily through stomatal pores. Here, we report that an Oryza sativa half-size ATP-binding cassette (ABC) subfamily G protein, RCN 1/OsABCG5, is involved in stomatal...

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Veröffentlicht in:Molecular plant 2016-03, Vol.9 (3), p.417-427
Hauptverfasser: Matsuda, Shuichi, Takano, Sho, Sato, Moeko, Furukawa, Kaoru, Nagasawa, Hidetaka, Yoshikawa, Shoko, Kasuga, Jun, Tokuji, Yoshihiko, Yazaki, Kazufumi, Nakazono, Mikio, Takamure, Itsuro, Kato, Kiyoaki
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container_end_page 427
container_issue 3
container_start_page 417
container_title Molecular plant
container_volume 9
creator Matsuda, Shuichi
Takano, Sho
Sato, Moeko
Furukawa, Kaoru
Nagasawa, Hidetaka
Yoshikawa, Shoko
Kasuga, Jun
Tokuji, Yoshihiko
Yazaki, Kazufumi
Nakazono, Mikio
Takamure, Itsuro
Kato, Kiyoaki
description Water stress is one of the major environmental stresses that affect agricultural production worldwide. Water loss from plants occurs primarily through stomatal pores. Here, we report that an Oryza sativa half-size ATP-binding cassette (ABC) subfamily G protein, RCN 1/OsABCG5, is involved in stomatal closure mediated by phytohormone abscisic acid (ABA) accumulation in guard cells. We found that the GFP-RCN1/ OsABCG5-fusion protein was localized at the plasma membrane in guard cells. The percentage of guard cell pairs containing both ABA and GFP-RCN1/OsABCG5 increased after exogenous ABA treatment, whereas they were co-localized in guard cell pairs regardless of whether exogenous ABA was applied. ABA application resulted in a smaller increase in the percentage of guard cell pairs containing ABA in rcnl mutant (A684P) and RCN1-RNAi than in wild-type plants. Furthermore, polyethylene glycol (drought stress)-inducible ABA accumulation in guard cells did not occur in rcnl mutants. Stomata closure mediated by exogenous ABA application was strongly reduced in rcnl mutants. Finally, rcnl mutant plants had more rapid water loss from detached leaves than the wild-type plants. These results indicate that in response to drought stress, RCN1/OsABCG5 is involved in accumulation of ABA in guard cells, which is indispensable for stomatal closure.
doi_str_mv 10.1016/j.molp.2015.12.007
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Water loss from plants occurs primarily through stomatal pores. Here, we report that an Oryza sativa half-size ATP-binding cassette (ABC) subfamily G protein, RCN 1/OsABCG5, is involved in stomatal closure mediated by phytohormone abscisic acid (ABA) accumulation in guard cells. We found that the GFP-RCN1/ OsABCG5-fusion protein was localized at the plasma membrane in guard cells. The percentage of guard cell pairs containing both ABA and GFP-RCN1/OsABCG5 increased after exogenous ABA treatment, whereas they were co-localized in guard cell pairs regardless of whether exogenous ABA was applied. ABA application resulted in a smaller increase in the percentage of guard cell pairs containing ABA in rcnl mutant (A684P) and RCN1-RNAi than in wild-type plants. Furthermore, polyethylene glycol (drought stress)-inducible ABA accumulation in guard cells did not occur in rcnl mutants. Stomata closure mediated by exogenous ABA application was strongly reduced in rcnl mutants. 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subjects ABA积累
ABC transporter
abscisic acid
ATP
ATP Binding Cassette Transporter, Sub-Family G, Member 5 - chemistry
ATP Binding Cassette Transporter, Sub-Family G, Member 5 - genetics
ATP Binding Cassette Transporter, Sub-Family G, Member 5 - metabolism
Cell Membrane - metabolism
drought stress
Droughts
Mutation
Oryza - anatomy & histology
Oryza - cytology
Oryza - metabolism
Oryza - physiology
Plant Proteins - chemistry
Plant Proteins - genetics
Plant Proteins - metabolism
Plant Stomata - anatomy & histology
Protein Multimerization
Protein Structure, Quaternary
Protein Transport
rice
stomatal closure
Stress, Physiological
Up-Regulation
保卫细胞
外源ABA
植物水分
气孔关闭
细胞质膜
转运体
title Rice Stomatal Closure Requires Guard Cell Plasma Membrane ATP-Binding Cassette Transporter RCN1/OsABCG5
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