Rice Stomatal Closure Requires Guard Cell Plasma Membrane ATP-Binding Cassette Transporter RCN1/OsABCG5
Water stress is one of the major environmental stresses that affect agricultural production worldwide. Water loss from plants occurs primarily through stomatal pores. Here, we report that an Oryza sativa half-size ATP-binding cassette (ABC) subfamily G protein, RCN 1/OsABCG5, is involved in stomatal...
Gespeichert in:
Veröffentlicht in: | Molecular plant 2016-03, Vol.9 (3), p.417-427 |
---|---|
Hauptverfasser: | , , , , , , , , , , , |
Format: | Artikel |
Sprache: | eng |
Schlagworte: | |
Online-Zugang: | Volltext |
Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
container_end_page | 427 |
---|---|
container_issue | 3 |
container_start_page | 417 |
container_title | Molecular plant |
container_volume | 9 |
creator | Matsuda, Shuichi Takano, Sho Sato, Moeko Furukawa, Kaoru Nagasawa, Hidetaka Yoshikawa, Shoko Kasuga, Jun Tokuji, Yoshihiko Yazaki, Kazufumi Nakazono, Mikio Takamure, Itsuro Kato, Kiyoaki |
description | Water stress is one of the major environmental stresses that affect agricultural production worldwide. Water loss from plants occurs primarily through stomatal pores. Here, we report that an Oryza sativa half-size ATP-binding cassette (ABC) subfamily G protein, RCN 1/OsABCG5, is involved in stomatal closure mediated by phytohormone abscisic acid (ABA) accumulation in guard cells. We found that the GFP-RCN1/ OsABCG5-fusion protein was localized at the plasma membrane in guard cells. The percentage of guard cell pairs containing both ABA and GFP-RCN1/OsABCG5 increased after exogenous ABA treatment, whereas they were co-localized in guard cell pairs regardless of whether exogenous ABA was applied. ABA application resulted in a smaller increase in the percentage of guard cell pairs containing ABA in rcnl mutant (A684P) and RCN1-RNAi than in wild-type plants. Furthermore, polyethylene glycol (drought stress)-inducible ABA accumulation in guard cells did not occur in rcnl mutants. Stomata closure mediated by exogenous ABA application was strongly reduced in rcnl mutants. Finally, rcnl mutant plants had more rapid water loss from detached leaves than the wild-type plants. These results indicate that in response to drought stress, RCN1/OsABCG5 is involved in accumulation of ABA in guard cells, which is indispensable for stomatal closure. |
doi_str_mv | 10.1016/j.molp.2015.12.007 |
format | Article |
fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_1772149082</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><cqvip_id>669433314</cqvip_id><els_id>S1674205215004608</els_id><sourcerecordid>1772149082</sourcerecordid><originalsourceid>FETCH-LOGICAL-c427t-312eaa767e62d8020b1ff9a0f325d6d9f2514aac60cad7e27c23e17df2846e9a3</originalsourceid><addsrcrecordid>eNp9kEFv1DAQhSNERUvhD3BAFicuSe1xYicSl21EF6RCq2U5W157sniVxLt2gsS_r1e79NjTjDRv3rz5suwDowWjTNzsisH3-wIoqwoGBaXyVXbFZAV5Uwv5OvVCljnQCi6ztzHuKBW0FvxNdglCpo5WV9l25QySX5Mf9KR70vY-zgHJCg-zCxjJctbBkhb7njz2Og6a_MBhE_SIZLF-zG_daN24Ja2OEacJyTqN4t6HCQNZtT_ZzUNc3LbL6l120ek-4vtzvc5-331dt9_y-4fl93Zxn5sS5JRzBqi1FBIF2JoC3bCuazTtOFRW2KaDipVaG0GNthJBGuDIpO2gLgU2ml9nn0----APM8ZJDS6aFD8l9nNUTEpgZUNrSFI4SU3wMQbs1D64QYd_ilF1BKx26ghYHQErBioBTksfz_7zZkD7vPKfaBJ8OQkwffnXYVDROBwN2sTTTMp697L_p3OoP37cHhLb5xNCNCXnnJX8CYRzlt8</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1772149082</pqid></control><display><type>article</type><title>Rice Stomatal Closure Requires Guard Cell Plasma Membrane ATP-Binding Cassette Transporter RCN1/OsABCG5</title><source>MEDLINE</source><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><source>Alma/SFX Local Collection</source><creator>Matsuda, Shuichi ; Takano, Sho ; Sato, Moeko ; Furukawa, Kaoru ; Nagasawa, Hidetaka ; Yoshikawa, Shoko ; Kasuga, Jun ; Tokuji, Yoshihiko ; Yazaki, Kazufumi ; Nakazono, Mikio ; Takamure, Itsuro ; Kato, Kiyoaki</creator><creatorcontrib>Matsuda, Shuichi ; Takano, Sho ; Sato, Moeko ; Furukawa, Kaoru ; Nagasawa, Hidetaka ; Yoshikawa, Shoko ; Kasuga, Jun ; Tokuji, Yoshihiko ; Yazaki, Kazufumi ; Nakazono, Mikio ; Takamure, Itsuro ; Kato, Kiyoaki</creatorcontrib><description>Water stress is one of the major environmental stresses that affect agricultural production worldwide. Water loss from plants occurs primarily through stomatal pores. Here, we report that an Oryza sativa half-size ATP-binding cassette (ABC) subfamily G protein, RCN 1/OsABCG5, is involved in stomatal closure mediated by phytohormone abscisic acid (ABA) accumulation in guard cells. We found that the GFP-RCN1/ OsABCG5-fusion protein was localized at the plasma membrane in guard cells. The percentage of guard cell pairs containing both ABA and GFP-RCN1/OsABCG5 increased after exogenous ABA treatment, whereas they were co-localized in guard cell pairs regardless of whether exogenous ABA was applied. ABA application resulted in a smaller increase in the percentage of guard cell pairs containing ABA in rcnl mutant (A684P) and RCN1-RNAi than in wild-type plants. Furthermore, polyethylene glycol (drought stress)-inducible ABA accumulation in guard cells did not occur in rcnl mutants. Stomata closure mediated by exogenous ABA application was strongly reduced in rcnl mutants. Finally, rcnl mutant plants had more rapid water loss from detached leaves than the wild-type plants. These results indicate that in response to drought stress, RCN1/OsABCG5 is involved in accumulation of ABA in guard cells, which is indispensable for stomatal closure.</description><identifier>ISSN: 1674-2052</identifier><identifier>EISSN: 1752-9867</identifier><identifier>DOI: 10.1016/j.molp.2015.12.007</identifier><identifier>PMID: 26708605</identifier><language>eng</language><publisher>England: Elsevier Inc</publisher><subject>ABA积累 ; ABC transporter ; abscisic acid ; ATP ; ATP Binding Cassette Transporter, Sub-Family G, Member 5 - chemistry ; ATP Binding Cassette Transporter, Sub-Family G, Member 5 - genetics ; ATP Binding Cassette Transporter, Sub-Family G, Member 5 - metabolism ; Cell Membrane - metabolism ; drought stress ; Droughts ; Mutation ; Oryza - anatomy & histology ; Oryza - cytology ; Oryza - metabolism ; Oryza - physiology ; Plant Proteins - chemistry ; Plant Proteins - genetics ; Plant Proteins - metabolism ; Plant Stomata - anatomy & histology ; Protein Multimerization ; Protein Structure, Quaternary ; Protein Transport ; rice ; stomatal closure ; Stress, Physiological ; Up-Regulation ; 保卫细胞 ; 外源ABA ; 植物水分 ; 气孔关闭 ; 细胞质膜 ; 转运体</subject><ispartof>Molecular plant, 2016-03, Vol.9 (3), p.417-427</ispartof><rights>2016 The Author</rights><rights>Copyright © 2016 The Author. Published by Elsevier Inc. All rights reserved.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c427t-312eaa767e62d8020b1ff9a0f325d6d9f2514aac60cad7e27c23e17df2846e9a3</citedby><cites>FETCH-LOGICAL-c427t-312eaa767e62d8020b1ff9a0f325d6d9f2514aac60cad7e27c23e17df2846e9a3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://image.cqvip.com/vip1000/qk/90143B/90143B.jpg</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/26708605$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Matsuda, Shuichi</creatorcontrib><creatorcontrib>Takano, Sho</creatorcontrib><creatorcontrib>Sato, Moeko</creatorcontrib><creatorcontrib>Furukawa, Kaoru</creatorcontrib><creatorcontrib>Nagasawa, Hidetaka</creatorcontrib><creatorcontrib>Yoshikawa, Shoko</creatorcontrib><creatorcontrib>Kasuga, Jun</creatorcontrib><creatorcontrib>Tokuji, Yoshihiko</creatorcontrib><creatorcontrib>Yazaki, Kazufumi</creatorcontrib><creatorcontrib>Nakazono, Mikio</creatorcontrib><creatorcontrib>Takamure, Itsuro</creatorcontrib><creatorcontrib>Kato, Kiyoaki</creatorcontrib><title>Rice Stomatal Closure Requires Guard Cell Plasma Membrane ATP-Binding Cassette Transporter RCN1/OsABCG5</title><title>Molecular plant</title><addtitle>Molecular Plant</addtitle><description>Water stress is one of the major environmental stresses that affect agricultural production worldwide. Water loss from plants occurs primarily through stomatal pores. Here, we report that an Oryza sativa half-size ATP-binding cassette (ABC) subfamily G protein, RCN 1/OsABCG5, is involved in stomatal closure mediated by phytohormone abscisic acid (ABA) accumulation in guard cells. We found that the GFP-RCN1/ OsABCG5-fusion protein was localized at the plasma membrane in guard cells. The percentage of guard cell pairs containing both ABA and GFP-RCN1/OsABCG5 increased after exogenous ABA treatment, whereas they were co-localized in guard cell pairs regardless of whether exogenous ABA was applied. ABA application resulted in a smaller increase in the percentage of guard cell pairs containing ABA in rcnl mutant (A684P) and RCN1-RNAi than in wild-type plants. Furthermore, polyethylene glycol (drought stress)-inducible ABA accumulation in guard cells did not occur in rcnl mutants. Stomata closure mediated by exogenous ABA application was strongly reduced in rcnl mutants. Finally, rcnl mutant plants had more rapid water loss from detached leaves than the wild-type plants. These results indicate that in response to drought stress, RCN1/OsABCG5 is involved in accumulation of ABA in guard cells, which is indispensable for stomatal closure.</description><subject>ABA积累</subject><subject>ABC transporter</subject><subject>abscisic acid</subject><subject>ATP</subject><subject>ATP Binding Cassette Transporter, Sub-Family G, Member 5 - chemistry</subject><subject>ATP Binding Cassette Transporter, Sub-Family G, Member 5 - genetics</subject><subject>ATP Binding Cassette Transporter, Sub-Family G, Member 5 - metabolism</subject><subject>Cell Membrane - metabolism</subject><subject>drought stress</subject><subject>Droughts</subject><subject>Mutation</subject><subject>Oryza - anatomy & histology</subject><subject>Oryza - cytology</subject><subject>Oryza - metabolism</subject><subject>Oryza - physiology</subject><subject>Plant Proteins - chemistry</subject><subject>Plant Proteins - genetics</subject><subject>Plant Proteins - metabolism</subject><subject>Plant Stomata - anatomy & histology</subject><subject>Protein Multimerization</subject><subject>Protein Structure, Quaternary</subject><subject>Protein Transport</subject><subject>rice</subject><subject>stomatal closure</subject><subject>Stress, Physiological</subject><subject>Up-Regulation</subject><subject>保卫细胞</subject><subject>外源ABA</subject><subject>植物水分</subject><subject>气孔关闭</subject><subject>细胞质膜</subject><subject>转运体</subject><issn>1674-2052</issn><issn>1752-9867</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kEFv1DAQhSNERUvhD3BAFicuSe1xYicSl21EF6RCq2U5W157sniVxLt2gsS_r1e79NjTjDRv3rz5suwDowWjTNzsisH3-wIoqwoGBaXyVXbFZAV5Uwv5OvVCljnQCi6ztzHuKBW0FvxNdglCpo5WV9l25QySX5Mf9KR70vY-zgHJCg-zCxjJctbBkhb7njz2Og6a_MBhE_SIZLF-zG_daN24Ja2OEacJyTqN4t6HCQNZtT_ZzUNc3LbL6l120ek-4vtzvc5-331dt9_y-4fl93Zxn5sS5JRzBqi1FBIF2JoC3bCuazTtOFRW2KaDipVaG0GNthJBGuDIpO2gLgU2ml9nn0----APM8ZJDS6aFD8l9nNUTEpgZUNrSFI4SU3wMQbs1D64QYd_ilF1BKx26ghYHQErBioBTksfz_7zZkD7vPKfaBJ8OQkwffnXYVDROBwN2sTTTMp697L_p3OoP37cHhLb5xNCNCXnnJX8CYRzlt8</recordid><startdate>20160307</startdate><enddate>20160307</enddate><creator>Matsuda, Shuichi</creator><creator>Takano, Sho</creator><creator>Sato, Moeko</creator><creator>Furukawa, Kaoru</creator><creator>Nagasawa, Hidetaka</creator><creator>Yoshikawa, Shoko</creator><creator>Kasuga, Jun</creator><creator>Tokuji, Yoshihiko</creator><creator>Yazaki, Kazufumi</creator><creator>Nakazono, Mikio</creator><creator>Takamure, Itsuro</creator><creator>Kato, Kiyoaki</creator><general>Elsevier Inc</general><scope>2RA</scope><scope>92L</scope><scope>CQIGP</scope><scope>W94</scope><scope>WU4</scope><scope>~WA</scope><scope>6I.</scope><scope>AAFTH</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20160307</creationdate><title>Rice Stomatal Closure Requires Guard Cell Plasma Membrane ATP-Binding Cassette Transporter RCN1/OsABCG5</title><author>Matsuda, Shuichi ; Takano, Sho ; Sato, Moeko ; Furukawa, Kaoru ; Nagasawa, Hidetaka ; Yoshikawa, Shoko ; Kasuga, Jun ; Tokuji, Yoshihiko ; Yazaki, Kazufumi ; Nakazono, Mikio ; Takamure, Itsuro ; Kato, Kiyoaki</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c427t-312eaa767e62d8020b1ff9a0f325d6d9f2514aac60cad7e27c23e17df2846e9a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>ABA积累</topic><topic>ABC transporter</topic><topic>abscisic acid</topic><topic>ATP</topic><topic>ATP Binding Cassette Transporter, Sub-Family G, Member 5 - chemistry</topic><topic>ATP Binding Cassette Transporter, Sub-Family G, Member 5 - genetics</topic><topic>ATP Binding Cassette Transporter, Sub-Family G, Member 5 - metabolism</topic><topic>Cell Membrane - metabolism</topic><topic>drought stress</topic><topic>Droughts</topic><topic>Mutation</topic><topic>Oryza - anatomy & histology</topic><topic>Oryza - cytology</topic><topic>Oryza - metabolism</topic><topic>Oryza - physiology</topic><topic>Plant Proteins - chemistry</topic><topic>Plant Proteins - genetics</topic><topic>Plant Proteins - metabolism</topic><topic>Plant Stomata - anatomy & histology</topic><topic>Protein Multimerization</topic><topic>Protein Structure, Quaternary</topic><topic>Protein Transport</topic><topic>rice</topic><topic>stomatal closure</topic><topic>Stress, Physiological</topic><topic>Up-Regulation</topic><topic>保卫细胞</topic><topic>外源ABA</topic><topic>植物水分</topic><topic>气孔关闭</topic><topic>细胞质膜</topic><topic>转运体</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Matsuda, Shuichi</creatorcontrib><creatorcontrib>Takano, Sho</creatorcontrib><creatorcontrib>Sato, Moeko</creatorcontrib><creatorcontrib>Furukawa, Kaoru</creatorcontrib><creatorcontrib>Nagasawa, Hidetaka</creatorcontrib><creatorcontrib>Yoshikawa, Shoko</creatorcontrib><creatorcontrib>Kasuga, Jun</creatorcontrib><creatorcontrib>Tokuji, Yoshihiko</creatorcontrib><creatorcontrib>Yazaki, Kazufumi</creatorcontrib><creatorcontrib>Nakazono, Mikio</creatorcontrib><creatorcontrib>Takamure, Itsuro</creatorcontrib><creatorcontrib>Kato, Kiyoaki</creatorcontrib><collection>中文科技期刊数据库</collection><collection>中文科技期刊数据库-CALIS站点</collection><collection>中文科技期刊数据库-7.0平台</collection><collection>中文科技期刊数据库-自然科学</collection><collection>中文科技期刊数据库-自然科学-生物科学</collection><collection>中文科技期刊数据库- 镜像站点</collection><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Molecular plant</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Matsuda, Shuichi</au><au>Takano, Sho</au><au>Sato, Moeko</au><au>Furukawa, Kaoru</au><au>Nagasawa, Hidetaka</au><au>Yoshikawa, Shoko</au><au>Kasuga, Jun</au><au>Tokuji, Yoshihiko</au><au>Yazaki, Kazufumi</au><au>Nakazono, Mikio</au><au>Takamure, Itsuro</au><au>Kato, Kiyoaki</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Rice Stomatal Closure Requires Guard Cell Plasma Membrane ATP-Binding Cassette Transporter RCN1/OsABCG5</atitle><jtitle>Molecular plant</jtitle><addtitle>Molecular Plant</addtitle><date>2016-03-07</date><risdate>2016</risdate><volume>9</volume><issue>3</issue><spage>417</spage><epage>427</epage><pages>417-427</pages><issn>1674-2052</issn><eissn>1752-9867</eissn><abstract>Water stress is one of the major environmental stresses that affect agricultural production worldwide. Water loss from plants occurs primarily through stomatal pores. Here, we report that an Oryza sativa half-size ATP-binding cassette (ABC) subfamily G protein, RCN 1/OsABCG5, is involved in stomatal closure mediated by phytohormone abscisic acid (ABA) accumulation in guard cells. We found that the GFP-RCN1/ OsABCG5-fusion protein was localized at the plasma membrane in guard cells. The percentage of guard cell pairs containing both ABA and GFP-RCN1/OsABCG5 increased after exogenous ABA treatment, whereas they were co-localized in guard cell pairs regardless of whether exogenous ABA was applied. ABA application resulted in a smaller increase in the percentage of guard cell pairs containing ABA in rcnl mutant (A684P) and RCN1-RNAi than in wild-type plants. Furthermore, polyethylene glycol (drought stress)-inducible ABA accumulation in guard cells did not occur in rcnl mutants. Stomata closure mediated by exogenous ABA application was strongly reduced in rcnl mutants. Finally, rcnl mutant plants had more rapid water loss from detached leaves than the wild-type plants. These results indicate that in response to drought stress, RCN1/OsABCG5 is involved in accumulation of ABA in guard cells, which is indispensable for stomatal closure.</abstract><cop>England</cop><pub>Elsevier Inc</pub><pmid>26708605</pmid><doi>10.1016/j.molp.2015.12.007</doi><tpages>11</tpages><oa>free_for_read</oa></addata></record> |
fulltext | fulltext |
identifier | ISSN: 1674-2052 |
ispartof | Molecular plant, 2016-03, Vol.9 (3), p.417-427 |
issn | 1674-2052 1752-9867 |
language | eng |
recordid | cdi_proquest_miscellaneous_1772149082 |
source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Alma/SFX Local Collection |
subjects | ABA积累 ABC transporter abscisic acid ATP ATP Binding Cassette Transporter, Sub-Family G, Member 5 - chemistry ATP Binding Cassette Transporter, Sub-Family G, Member 5 - genetics ATP Binding Cassette Transporter, Sub-Family G, Member 5 - metabolism Cell Membrane - metabolism drought stress Droughts Mutation Oryza - anatomy & histology Oryza - cytology Oryza - metabolism Oryza - physiology Plant Proteins - chemistry Plant Proteins - genetics Plant Proteins - metabolism Plant Stomata - anatomy & histology Protein Multimerization Protein Structure, Quaternary Protein Transport rice stomatal closure Stress, Physiological Up-Regulation 保卫细胞 外源ABA 植物水分 气孔关闭 细胞质膜 转运体 |
title | Rice Stomatal Closure Requires Guard Cell Plasma Membrane ATP-Binding Cassette Transporter RCN1/OsABCG5 |
url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-23T16%3A12%3A32IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Rice%20Stomatal%20Closure%20Requires%20Guard%20Cell%20Plasma%20Membrane%20ATP-Binding%20Cassette%20Transporter%20RCN1/OsABCG5&rft.jtitle=Molecular%20plant&rft.au=Matsuda,%20Shuichi&rft.date=2016-03-07&rft.volume=9&rft.issue=3&rft.spage=417&rft.epage=427&rft.pages=417-427&rft.issn=1674-2052&rft.eissn=1752-9867&rft_id=info:doi/10.1016/j.molp.2015.12.007&rft_dat=%3Cproquest_cross%3E1772149082%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1772149082&rft_id=info:pmid/26708605&rft_cqvip_id=669433314&rft_els_id=S1674205215004608&rfr_iscdi=true |