Detection of genes essential in specific niches by signature-tagged mutagenesis

Detection of genes essential in specific niches by signature-tagged mutagenesis

Variations of the signature-tagged mutagenesis (STM) technique are now possible and the method can be applied to most pathogens that have an STM-selectable phenotype in a host system. STM screening of 15,040 mutants from 11 bacterial species identified 323 in vivo attenuated mutants. As a genome-sca...

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Veröffentlicht in:Current Opinion in Biotechnology 2000-10, Vol.11 (5), p.434-439
Hauptverfasser: Lehoux, Dario E, Levesque, Roger C
Format: Artikel
Sprache:eng
Schlagworte:
Animals
Bacteria - pathogenicity
Fungi - pathogenicity
Genetic Techniques
Mutagenesis, Insertional - methods
Parasites - pathogenicity
Virulence - genetics
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Zusammenfassung:Variations of the signature-tagged mutagenesis (STM) technique are now possible and the method can be applied to most pathogens that have an STM-selectable phenotype in a host system. STM screening of 15,040 mutants from 11 bacterial species identified 323 in vivo attenuated mutants. As a genome-scanning tool, STM will yield information about genes with unknown functions as well as information crucial for understanding microbial pathogenesis.
ISSN:0958-1669
1879-0429
DOI:10.1016/S0958-1669(00)00124-5