The Vav Exchange Factor Is an Essential Regulator in Actin-Dependent Receptor Translocation to the Lymphocyte-Antigen-Presenting Cell Interface
During the interaction of a T cell with an antigen-presenting cell (APC), several receptor ligand pairs, including the T cell receptor (TCR)/major histocompatibility complex (MHC), accumulate at the T cell/APC interface in defined geometrical patterns. This accumulation depends on a movement of the...
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Veröffentlicht in: | Proceedings of the National Academy of Sciences - PNAS 2000-08, Vol.97 (18), p.10150-10155 |
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Zusammenfassung: | During the interaction of a T cell with an antigen-presenting cell (APC), several receptor ligand pairs, including the T cell receptor (TCR)/major histocompatibility complex (MHC), accumulate at the T cell/APC interface in defined geometrical patterns. This accumulation depends on a movement of the T cell cortical actin cytoskeleton toward the interface. Here we study the involvement of the guanine nucleotide exchange factor vav in this process. We crossed 129 vav-/-mice with B10/BR 5C.C7 TCR transgenic mice and used peptide-loaded APCs to stimulate T cells from the offspring. We found that the accumulation of TCR/MHC at the T cell/APC interface and the T cell actin cytoskeleton rearrangement were clearly defective in these vav+/-mice. A comparable defect in superantigen-mediated T cell activation of T cells from non-TCR transgenic 129 mice was also observed, although in this case it was more apparent in vav-/-mice. These data indicate that vav is an essential regulator of cytoskeletal rearrangements during T cell activation. |
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ISSN: | 0027-8424 1091-6490 |
DOI: | 10.1073/pnas.97.18.10150 |