In situ characterization of resin–dentin interfaces using conventional vs. cryofocused ion-beam milling

Highlights • Cryo-FIB specimen preparation for TEM imaging of resin–dentin interfaces is proposed. • We report step by step FIB sample preparations in details. • Minimizing the milling temperature improve collagen fibrils visibility.

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Bibliographische Detailangaben
Veröffentlicht in:	Dental materials 2015-07, Vol.31 (7), p.833-844
Hauptverfasser:	Bakhsh, Turki A, Sadr, Alireza, Mandurah, Mona M, Shimada, Yasushi, Zakaria, Osama, Tagami, Junji
Format:	Artikel
Sprache:	eng
Schlagworte:	Advanced Basic Science Antimony Bicuspid Collagen Composite Resins - chemistry Cryo Cryogenic cooling Dental adhesive Dental Bonding Dental Cavity Preparation - methods Dental materials Dental Materials - chemistry Dentin Dentin - chemistry Dentin-Bonding Agents - chemistry Dentistry FIB Hybrid layer Ion beams Lead (metal) Microscopy, Confocal Microscopy, Electron, Transmission Resin composite Resins, Synthetic - chemistry Silorane Resins - chemistry Surface Properties Teeth Teeth, dentin, nano-interaction zone TEM Temperature Transmission electron microscopy
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Beschreibung
Zusammenfassung:	Highlights • Cryo-FIB specimen preparation for TEM imaging of resin–dentin interfaces is proposed. • We report step by step FIB sample preparations in details. • Minimizing the milling temperature improve collagen fibrils visibility.
ISSN:	0109-5641 1879-0097
DOI:	10.1016/j.dental.2015.04.010