Study of molecule variations in human xanthelasma skin based on confocal micro-raman spectroscopy

Summary Confocal micro‐Raman spectroscopy was used to distinguish human xanthelasma skin (HXS) from the human normal skin (HNS). Results showed that intensive Raman peaks at 1,269, 1,336, 1,448, and 1,656 cm−1 increased obviously. Both 1,269 and 1,656 cm−1 peaks showed that the proteins in HXS were mostly in the anti‐parallel ß sheet conformation. While the intensities of bands at 1,032, 1,087, 1,300, and 1,448 cm−1 belonged to lipids were enhanced in HXS spectrum compared to those in HNS spectrum. There were main intercellular lipids alkyl chains with minor proteins contribution at 1,087 cm−1 and phenylalanine at 1,032 cm−1. To quantitative analysis of the difference, the ratio of I852/I829 was calculated, which was 1:1.04 ± 0.04 and 1:1.11 ± 0.02 for HNS and HXS (p