Assessment of HEMA and TEGDMA induced DNA damage by multiple genotoxicological endpoints in human lymphocytes

Highlights • Human lymphocytes were exposed to HEMA and TEGDMA for 24 h. • Cytotoxic effects were measured only in TEGDMA (100 μM) treated cells. • Genotoxic effects were detected in the comet assay at the highest concentrations. • No increase in the micronucleus frequency or an arrest of the cell c...

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Veröffentlicht in:	Dental materials 2015-08, Vol.31 (8), p.865-876
Hauptverfasser:	Ginzkey, Christian, Zinnitsch, Sabrina, Steussloff, Gudrun, Koehler, Christian, Hackenberg, Stephan, Hagen, Rudolf, Kleinsasser, Norbert H, Froelich, Katrin
Format:	Artikel
Sprache:	eng
Schlagworte:	Advanced Basic Science Apoptosis - drug effects Bioassay Cell Survival - drug effects Chromosome aberration Chromosome Aberrations Comet Assay Cytotoxicity Damage Dentistry DNA Damage - drug effects Flow Cytometry Genotoxicity HEMA Human lymphocytes Humans Lymphocytes - drug effects Methacrylates - toxicity Monomers Polyethylene Glycols - toxicity Polymers Polymethacrylic Acids - toxicity Resins Sister Chromatid Exchange TEGDMA Triethylene glycol dimethacrylate Viability
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Zusammenfassung:	Highlights • Human lymphocytes were exposed to HEMA and TEGDMA for 24 h. • Cytotoxic effects were measured only in TEGDMA (100 μM) treated cells. • Genotoxic effects were detected in the comet assay at the highest concentrations. • No increase in the micronucleus frequency or an arrest of the cell cycle was shown. • Chromosome aberrations and SCEs were recorded at lowest concentrations tested.
ISSN:	0109-5641 1879-0097
DOI:	10.1016/j.dental.2015.04.009