Brix, pH and anthocyanin content determination in whole Port wine grape berries by hyperspectral imaging and neural networks

•Concurrent measurement of pH, sugars and anthocyanin content from hyperspectral data.•Measurement of enological parameters in samples composed of six whole grape berries.•Very few works in scientific literature use samples with such small number of berries.•Highly competitive results for pH and sugars for reflectance and transmittance modes.•Major advance of the state-of-the-art for anthocyanin content also for both modes. This work presents the results of measuring pH, sugars, and anthocyanin content of whole grape berries. The spectrum of each sample, composed of six whole grape berries, was collected using hyperspectral imaging in reflectance mode from 380 to 1028nm. The spectra were converted to enological parameters by multilayer perceptrons created using 240 samples that were split for 7-fold cross-validation and test. The test set with 30 samples revealed R2 values of 0.73, 0.92 and 0.95 and RMSE of 0.18, 0.95°Brix and 14mg/l for pH, sugars and anthocyanin content, respectively. This is the only work, up to the authors’ knowledge, that reports the simultaneous determination of pH, sugars and anthocyanin with a spatial resolution smaller than the bunch size, by spectroscopy measurements done in reflectance mode. The results presented show an improvement of the state-of-the-art, for both reflectance and transmittance mode, that is particularly notorious for anthocyanin measurement.