Cheek blood sampling of mice

To explore the method of cheek blood sampling in mice in continuous blood test. Cheek blood sampling was carried out in 10 9-week-old mice once every 2-3 days. The volumes of blood samples were recorded. Reactions of the mice were observed. Cheek blood sampling collected 0.3-0.6 ml of blood every time, and did not affect the normal activities of the mice. The blood sampling could be repeated every 2-3 days. Cheek blood sampling has several advantages including being capable to draw adequate blood, simple and easy to operate, well tolerated, and ethical in treating laboratory animals.