Recombinant M2e outer membrane vesicle vaccines protect against lethal influenza A challenge in BALB/c mice

Highlights • Engineered probiotic E. coli Nissle 1917 express and present influenza M2e peptides in outer membrane vesicles (M2e-OMVs). • M2e-OMVs trigger the innate immune response predominantly through TLR2, TLR4, and TLR5 agonist activity. • BALB/c mice vaccinated with M2e-OMVs are 100% protected...

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Veröffentlicht in:	Vaccine 2016-03, Vol.34 (10), p.1252-1258
Hauptverfasser:	Rappazzo, C. Garrett, Watkins, Hannah C, Guarino, Cassandra M, Chau, Annie, Lopez, Jody L, DeLisa, Matthew P, Leifer, Cynthia A, Whittaker, Gary R, Putnam, David
Format:	Artikel
Sprache:	eng
Schlagworte:	Allergy and Immunology Animals Antibodies, Viral - blood Antigens Bias Conflicts of interest Drug dosages E coli Escherichia coli - metabolism Extracellular Vesicles - immunology Female Immune response Immunity, Innate Immunoglobulin G - blood Immunoglobulins Influenza A Virus, H1N1 Subtype Influenza Vaccines - immunology M2e subunit vaccines Mice, Inbred BALB C Nanoparticles Orthomyxoviridae Infections - prevention & control Outer membrane vesicles Pandemics Peptides Proteins Scholarships & fellowships TLR agonists Toll-Like Receptors - agonists Universal influenza vaccine Vaccines Vaccines, Synthetic - immunology Viral Matrix Proteins - immunology Viruses
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Zusammenfassung:	Highlights • Engineered probiotic E. coli Nissle 1917 express and present influenza M2e peptides in outer membrane vesicles (M2e-OMVs). • M2e-OMVs trigger the innate immune response predominantly through TLR2, TLR4, and TLR5 agonist activity. • BALB/c mice vaccinated with M2e-OMVs are 100% protected against a lethal dose of H1N1 influenza A virus.
ISSN:	0264-410X 1873-2518
DOI:	10.1016/j.vaccine.2016.01.028