Application of Molecular Diagnostics in Primary Detection of ESBL Directly from Clinical Specimens
The infections caused by extended-spectrum β-lactamase (ESBL)-producing organisms are associated with increased mortality. The real-time polymerase chain reaction (PCR) method, which enables detection of ESBLs directly from patients' clinical material, was developed. This study focused on bla C...
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Veröffentlicht in: | Microbial drug resistance (Larchmont, N.Y.) N.Y.), 2015-06, Vol.21 (3), p.352-357 |
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Format: | Artikel |
Sprache: | eng |
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Zusammenfassung: | The infections caused by extended-spectrum β-lactamase (ESBL)-producing organisms are associated with increased mortality. The real-time polymerase chain reaction (PCR) method, which enables detection of ESBLs directly from patients' clinical material, was developed. This study focused on
bla
CTX-M
and
bla
SHV
determination in endotracheal aspirates. Each sample was identified with standard microbiological procedures and simultaneously analyzed for the presence of nucleic acids, which encode CTX-M and SHV ESBL enzymes using real-time PCR. A total of 341 samples were investigated. In the set, 27 ESBL-positive samples were identified by phenotypic methods, while 60 positive samples were identified by the PCR method. Of the 60 PCR-positive samples, 58 were positive for the
bla
CTX-M
. In two samples, the ESBL
bla
SHV-ESBL
gene was detected. One phenotypically positive sample was PCR negative. The real-time PCR assay does not require a cultivation step and therefore enables detection of ESBL in 6 hours. The rapid method is necessary for early and adequate antimicrobial treatment. |
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ISSN: | 1076-6294 1931-8448 |
DOI: | 10.1089/mdr.2014.0210 |