Structural confirmation of novel oligosaccharides isolated from sugar beet molasses

•Eleven oligosaccharides were isolated from sugar beet molasses.•The structures of the oligosaccharides were confirmed using NMR techniques.•Three of 11 oligosaccharides were novel. Eleven oligosaccharides were isolated from sugar beet molasses using carbon–Celite column chromatography and HPLC. The constituent sugars and linkage positions were determined using methylation analysis, MALDI–TOF-MS, and NMR measurements. The configurations of isolated oligosaccharides were confirmed based on detailed NMR analysis. Based on our results, three of the 11 oligosaccharides were novel.