NuMA Phosphorylation by Aurora-A Orchestrates Spindle Orientation

Spindle positioning is essential for tissue morphogenesis and homeostasis. The signaling network synchronizing spindle placement with mitotic progression relies on timely recruitment at the cell cortex of NuMA:LGN:Gαi complexes, in which NuMA acts as a receptor for the microtubule motor Dynein. To study the implication of Aurora-A in spindle orientation, we developed protocols for the partial inhibition of its activity. Under these conditions, in metaphase NuMA and Dynein accumulate abnormally at the spindle poles and do not reach the cortex, while the cortical distribution of LGN remains unperturbed. FRAP experiments revealed that Aurora-A governs the dynamic exchange between the cytoplasmic and the spindle pole-localized pools of NuMA. We show that Aurora-A phosphorylates directly the C terminus of NuMA on three Ser residues, of which Ser1969 determines the dynamic behavior and the spindle orientation functions of NuMA. Most interestingly, we identify a new microtubule-binding domain of NuMA, which does not overlap with the LGN-binding motif. Our study demonstrates that in metaphase the direct phosphorylation of NuMA by Aurora-A controls its cortical enrichment, and that this is the major event underlying the spindle orientation functions of Aurora-A in transformed and non-transformed cells in culture. Phosphorylation of NuMA by Aurora-A does not affect its affinity for microtubules or for LGN but rather determines the mobility of the protein at the spindle poles. The finding that NuMA can associate concomitantly with LGN and microtubules suggests that its microtubule-binding activity contributes to anchor Dynein-loaded microtubule +TIPs at cortical sites with LGN. •Aurora-A contributes to spindle orientation by controlling NuMA cortical targeting•Phosphorylation of Ser1969NuMA by Aurora-A governs NuMA mobility at spindle poles•NuMA residues 2002–2115 bind to microtubules, independently of Aurora-A•NuMA binds simultaneously microtubules and the spindle orientation protein LGN Gallini et al. identify NuMA phosphorylation as the determinant for the Aurora-A spindle orientation function. In metaphase, Aurora-A governs the distribution of NuMA between poles and cortex by phosphorylating Ser1969NuMA, which controls NuMA mobility at poles. A novel microtubule-binding domain of NuMA is identified compatible with LGN binding.