Qualification of spontaneous undirected locomotor behavior of fish for sublethal toxicity testing. Part I. Variability of measurement parameters under general test conditions
An automated, personal computer–based video‐processing, object‐recognition, and object‐tracing system was used to record and analyze undirected spontaneous locomotor behavior of small groups of undisturbed semiadult zebra fish (Brachydanio rerio) in laboratory tanks. The primary data provided by the...
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Veröffentlicht in: | Environmental toxicology and chemistry 1999-12, Vol.18 (12), p.2736-2742 |
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Format: | Artikel |
Sprache: | eng |
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Zusammenfassung: | An automated, personal computer–based video‐processing, object‐recognition, and object‐tracing system was used to record and analyze undirected spontaneous locomotor behavior of small groups of undisturbed semiadult zebra fish (Brachydanio rerio) in laboratory tanks. The primary data provided by the monitoring system were the individually assigned, time‐stamped coordinates of the fish in two‐dimensional projection. Secondary parameters (position, velocity of movement in the horizontal and the vertical direction, and temporal intraindividual and interindividual association) were calculated. The computed parameters offered a multidimensional description of spontaneous undirected swimming behavior of the fish and proved to be largely independent of water temperature, length, weight, and sex ratio of the zebra fish within the standardized range, but varied significantly with the feeding regime, time of day, number of fish per tank, and batch. Statistical characteristics of the behavioral parameters confirmed them as being appropriate for parametric statistical analyses. |
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ISSN: | 0730-7268 1552-8618 |
DOI: | 10.1002/etc.5620181213 |