A method for isolating and culturing placental cells from failed early equine pregnancies
Abstract Early pregnancy loss occurs in 6–10% of equine pregnancies making it the main cause of reproductive wastage. Despite this, reasons for the losses are known in only 16% of cases. Lack of viable conceptus material has inhibited investigations of many potential genetic and pathological causes....
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| Veröffentlicht in: | Placenta (Eastbourne) 2016-02, Vol.38, p.107-111 |
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| creator | Rose, B.V Cabrera-Sharp, V Firth, M.J Barrelet, F.E Bate, S Cameron, I.J Crabtree, J.R Crowhurst, J McGladdery, A.J Neal, H Pynn, J Pynn, O.D Smith, C Wise, Z Verheyen, K.L.P Wathes, D.C de Mestre, A.M |
| description | Abstract Early pregnancy loss occurs in 6–10% of equine pregnancies making it the main cause of reproductive wastage. Despite this, reasons for the losses are known in only 16% of cases. Lack of viable conceptus material has inhibited investigations of many potential genetic and pathological causes. We present a method for isolating and culturing placental cells from failed early equine pregnancies. Trophoblast cells from 18/30 (60%) failed equine pregnancies of gestational ages 14–65 days were successfully cultured in three different media, with the greatest growth achieved for cells cultured in AmnioChrome™ Plus. Genomic DNA of a suitable quality for molecular assays was also isolated from 29/30 of these cases. This method will enable future investigations determining pathologies causing EPL. |
| doi_str_mv | 10.1016/j.placenta.2015.12.014 |
| format | Article |
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Despite this, reasons for the losses are known in only 16% of cases. Lack of viable conceptus material has inhibited investigations of many potential genetic and pathological causes. We present a method for isolating and culturing placental cells from failed early equine pregnancies. Trophoblast cells from 18/30 (60%) failed equine pregnancies of gestational ages 14–65 days were successfully cultured in three different media, with the greatest growth achieved for cells cultured in AmnioChrome™ Plus. Genomic DNA of a suitable quality for molecular assays was also isolated from 29/30 of these cases. This method will enable future investigations determining pathologies causing EPL.</description><identifier>ISSN: 0143-4004</identifier><identifier>EISSN: 1532-3102</identifier><identifier>DOI: 10.1016/j.placenta.2015.12.014</identifier><identifier>PMID: 26907389</identifier><language>eng</language><publisher>Netherlands: Elsevier Ltd</publisher><subject>Abortion, Veterinary - diagnostic imaging ; Abortion, Veterinary - pathology ; Animals ; Cell culture ; Cell Culture Techniques - methods ; Cell Culture Techniques - veterinary ; Cell Separation - methods ; Cells, Cultured ; Embryo Loss - diagnostic imaging ; Embryo Loss - pathology ; Embryo Loss - veterinary ; Embryonic loss ; Equine ; Female ; Gestational Age ; Horses ; Internal Medicine ; Obstetrics and Gynecology ; Placenta - diagnostic imaging ; Placenta - pathology ; Pregnancy ; Pregnancy loss ; Trophoblast ; Trophoblasts - pathology ; Ultrasonography, Prenatal - veterinary</subject><ispartof>Placenta (Eastbourne), 2016-02, Vol.38, p.107-111</ispartof><rights>Elsevier Ltd</rights><rights>2016 Elsevier Ltd</rights><rights>Copyright © 2016 Elsevier Ltd. All rights reserved.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c471t-92abc02d6ca0c28c2705ea42cd92349732979bdfac8fb97c28662eae39b2b8c93</citedby><cites>FETCH-LOGICAL-c471t-92abc02d6ca0c28c2705ea42cd92349732979bdfac8fb97c28662eae39b2b8c93</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0143400415301107$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65534</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/26907389$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Rose, B.V</creatorcontrib><creatorcontrib>Cabrera-Sharp, V</creatorcontrib><creatorcontrib>Firth, M.J</creatorcontrib><creatorcontrib>Barrelet, F.E</creatorcontrib><creatorcontrib>Bate, S</creatorcontrib><creatorcontrib>Cameron, I.J</creatorcontrib><creatorcontrib>Crabtree, J.R</creatorcontrib><creatorcontrib>Crowhurst, J</creatorcontrib><creatorcontrib>McGladdery, A.J</creatorcontrib><creatorcontrib>Neal, H</creatorcontrib><creatorcontrib>Pynn, J</creatorcontrib><creatorcontrib>Pynn, O.D</creatorcontrib><creatorcontrib>Smith, C</creatorcontrib><creatorcontrib>Wise, Z</creatorcontrib><creatorcontrib>Verheyen, K.L.P</creatorcontrib><creatorcontrib>Wathes, D.C</creatorcontrib><creatorcontrib>de Mestre, A.M</creatorcontrib><title>A method for isolating and culturing placental cells from failed early equine pregnancies</title><title>Placenta (Eastbourne)</title><addtitle>Placenta</addtitle><description>Abstract Early pregnancy loss occurs in 6–10% of equine pregnancies making it the main cause of reproductive wastage. Despite this, reasons for the losses are known in only 16% of cases. Lack of viable conceptus material has inhibited investigations of many potential genetic and pathological causes. We present a method for isolating and culturing placental cells from failed early equine pregnancies. Trophoblast cells from 18/30 (60%) failed equine pregnancies of gestational ages 14–65 days were successfully cultured in three different media, with the greatest growth achieved for cells cultured in AmnioChrome™ Plus. Genomic DNA of a suitable quality for molecular assays was also isolated from 29/30 of these cases. This method will enable future investigations determining pathologies causing EPL.</description><subject>Abortion, Veterinary - diagnostic imaging</subject><subject>Abortion, Veterinary - pathology</subject><subject>Animals</subject><subject>Cell culture</subject><subject>Cell Culture Techniques - methods</subject><subject>Cell Culture Techniques - veterinary</subject><subject>Cell Separation - methods</subject><subject>Cells, Cultured</subject><subject>Embryo Loss - diagnostic imaging</subject><subject>Embryo Loss - pathology</subject><subject>Embryo Loss - veterinary</subject><subject>Embryonic loss</subject><subject>Equine</subject><subject>Female</subject><subject>Gestational Age</subject><subject>Horses</subject><subject>Internal Medicine</subject><subject>Obstetrics and Gynecology</subject><subject>Placenta - diagnostic imaging</subject><subject>Placenta - pathology</subject><subject>Pregnancy</subject><subject>Pregnancy loss</subject><subject>Trophoblast</subject><subject>Trophoblasts - pathology</subject><subject>Ultrasonography, Prenatal - veterinary</subject><issn>0143-4004</issn><issn>1532-3102</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU9P3DAQxa0KVLbbfgXkI5ekHiebxBcEQv2DhMQBOPRkOfaEeuvYi51U2m9fR8v2wIWTNdZ782Z-Q8g5sBIYNF-35c4pjX5SJWewKYGXDOoPZAWbihcVMH5CVvmnKmrG6jPyKaUtY0zUwD-SM94I1ladWJFf13TE6XcwdAiR2hScmqx_psobqmc3zXGpjlmOanQu0SGGkQ7KOjQUVXR7ii-z9Uh3EZ-98tpi-kxOB-USfnl91-Tp-7fHm5_F3f2P25vru0LXLUyF4KrXjJtGK6Z5p3nLNqhqro3gVS3aiotW9GZQuht60WZJ03BUWIme950W1ZpcHPruYniZMU1ytGkZU3kMc5LQNh00reggS5uDVMeQUsRB7qIdVdxLYHLBKrfyuKpcsErgcoG4JuevGXM_ovlvO3LMgquDAPOmfy1GmTIDr9HYiHqSJtj3My7ftNDOequV-4N7TNswR585SpApG-TD4llumw_OAPIY_wCuMaIM</recordid><startdate>20160201</startdate><enddate>20160201</enddate><creator>Rose, B.V</creator><creator>Cabrera-Sharp, V</creator><creator>Firth, M.J</creator><creator>Barrelet, F.E</creator><creator>Bate, S</creator><creator>Cameron, I.J</creator><creator>Crabtree, J.R</creator><creator>Crowhurst, J</creator><creator>McGladdery, A.J</creator><creator>Neal, H</creator><creator>Pynn, J</creator><creator>Pynn, O.D</creator><creator>Smith, C</creator><creator>Wise, Z</creator><creator>Verheyen, K.L.P</creator><creator>Wathes, D.C</creator><creator>de Mestre, A.M</creator><general>Elsevier Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20160201</creationdate><title>A method for isolating and culturing placental cells from failed early equine pregnancies</title><author>Rose, B.V ; Cabrera-Sharp, V ; Firth, M.J ; Barrelet, F.E ; Bate, S ; Cameron, I.J ; Crabtree, J.R ; Crowhurst, J ; McGladdery, A.J ; Neal, H ; Pynn, J ; Pynn, O.D ; Smith, C ; Wise, Z ; Verheyen, K.L.P ; Wathes, D.C ; de Mestre, A.M</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c471t-92abc02d6ca0c28c2705ea42cd92349732979bdfac8fb97c28662eae39b2b8c93</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>Abortion, Veterinary - diagnostic imaging</topic><topic>Abortion, Veterinary - pathology</topic><topic>Animals</topic><topic>Cell culture</topic><topic>Cell Culture Techniques - methods</topic><topic>Cell Culture Techniques - veterinary</topic><topic>Cell Separation - methods</topic><topic>Cells, Cultured</topic><topic>Embryo Loss - diagnostic imaging</topic><topic>Embryo Loss - pathology</topic><topic>Embryo Loss - veterinary</topic><topic>Embryonic loss</topic><topic>Equine</topic><topic>Female</topic><topic>Gestational Age</topic><topic>Horses</topic><topic>Internal Medicine</topic><topic>Obstetrics and Gynecology</topic><topic>Placenta - diagnostic imaging</topic><topic>Placenta - pathology</topic><topic>Pregnancy</topic><topic>Pregnancy loss</topic><topic>Trophoblast</topic><topic>Trophoblasts - pathology</topic><topic>Ultrasonography, Prenatal - veterinary</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Rose, B.V</creatorcontrib><creatorcontrib>Cabrera-Sharp, V</creatorcontrib><creatorcontrib>Firth, M.J</creatorcontrib><creatorcontrib>Barrelet, F.E</creatorcontrib><creatorcontrib>Bate, S</creatorcontrib><creatorcontrib>Cameron, I.J</creatorcontrib><creatorcontrib>Crabtree, J.R</creatorcontrib><creatorcontrib>Crowhurst, J</creatorcontrib><creatorcontrib>McGladdery, A.J</creatorcontrib><creatorcontrib>Neal, H</creatorcontrib><creatorcontrib>Pynn, J</creatorcontrib><creatorcontrib>Pynn, O.D</creatorcontrib><creatorcontrib>Smith, C</creatorcontrib><creatorcontrib>Wise, Z</creatorcontrib><creatorcontrib>Verheyen, K.L.P</creatorcontrib><creatorcontrib>Wathes, D.C</creatorcontrib><creatorcontrib>de Mestre, A.M</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Placenta (Eastbourne)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Rose, B.V</au><au>Cabrera-Sharp, V</au><au>Firth, M.J</au><au>Barrelet, F.E</au><au>Bate, S</au><au>Cameron, I.J</au><au>Crabtree, J.R</au><au>Crowhurst, J</au><au>McGladdery, A.J</au><au>Neal, H</au><au>Pynn, J</au><au>Pynn, O.D</au><au>Smith, C</au><au>Wise, Z</au><au>Verheyen, K.L.P</au><au>Wathes, D.C</au><au>de Mestre, A.M</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A method for isolating and culturing placental cells from failed early equine pregnancies</atitle><jtitle>Placenta (Eastbourne)</jtitle><addtitle>Placenta</addtitle><date>2016-02-01</date><risdate>2016</risdate><volume>38</volume><spage>107</spage><epage>111</epage><pages>107-111</pages><issn>0143-4004</issn><eissn>1532-3102</eissn><abstract>Abstract Early pregnancy loss occurs in 6–10% of equine pregnancies making it the main cause of reproductive wastage. 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| ispartof | Placenta (Eastbourne), 2016-02, Vol.38, p.107-111 |
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| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals Complete |
| subjects | Abortion, Veterinary - diagnostic imaging Abortion, Veterinary - pathology Animals Cell culture Cell Culture Techniques - methods Cell Culture Techniques - veterinary Cell Separation - methods Cells, Cultured Embryo Loss - diagnostic imaging Embryo Loss - pathology Embryo Loss - veterinary Embryonic loss Equine Female Gestational Age Horses Internal Medicine Obstetrics and Gynecology Placenta - diagnostic imaging Placenta - pathology Pregnancy Pregnancy loss Trophoblast Trophoblasts - pathology Ultrasonography, Prenatal - veterinary |
| title | A method for isolating and culturing placental cells from failed early equine pregnancies |
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