A method for isolating and culturing placental cells from failed early equine pregnancies

Abstract Early pregnancy loss occurs in 6–10% of equine pregnancies making it the main cause of reproductive wastage. Despite this, reasons for the losses are known in only 16% of cases. Lack of viable conceptus material has inhibited investigations of many potential genetic and pathological causes....

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Veröffentlicht in:	Placenta (Eastbourne) 2016-02, Vol.38, p.107-111
Hauptverfasser:	Rose, B.V, Cabrera-Sharp, V, Firth, M.J, Barrelet, F.E, Bate, S, Cameron, I.J, Crabtree, J.R, Crowhurst, J, McGladdery, A.J, Neal, H, Pynn, J, Pynn, O.D, Smith, C, Wise, Z, Verheyen, K.L.P, Wathes, D.C, de Mestre, A.M
Format:	Artikel
Sprache:	eng
Schlagworte:	Abortion, Veterinary - diagnostic imaging Abortion, Veterinary - pathology Animals Cell culture Cell Culture Techniques - methods Cell Culture Techniques - veterinary Cell Separation - methods Cells, Cultured Embryo Loss - diagnostic imaging Embryo Loss - pathology Embryo Loss - veterinary Embryonic loss Equine Female Gestational Age Horses Internal Medicine Obstetrics and Gynecology Placenta - diagnostic imaging Placenta - pathology Pregnancy Pregnancy loss Trophoblast Trophoblasts - pathology Ultrasonography, Prenatal - veterinary
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Zusammenfassung:	Abstract Early pregnancy loss occurs in 6–10% of equine pregnancies making it the main cause of reproductive wastage. Despite this, reasons for the losses are known in only 16% of cases. Lack of viable conceptus material has inhibited investigations of many potential genetic and pathological causes. We present a method for isolating and culturing placental cells from failed early equine pregnancies. Trophoblast cells from 18/30 (60%) failed equine pregnancies of gestational ages 14–65 days were successfully cultured in three different media, with the greatest growth achieved for cells cultured in AmnioChrome™ Plus. Genomic DNA of a suitable quality for molecular assays was also isolated from 29/30 of these cases. This method will enable future investigations determining pathologies causing EPL.
ISSN:	0143-4004 1532-3102
DOI:	10.1016/j.placenta.2015.12.014