Differential proteome association study of freeze-thaw damage in ram sperm

In this study proteomics analysis was performed to investigate damage caused to ram sperm by the freeze-thaw process. Sperm motility, viability, reactive oxygen species (ROS) and adenosine triphosphate (ATP) content were measured to evaluate sperm quality. Compared with fresh groups, motility, viabi...

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Veröffentlicht in:	Cryobiology 2016-02, Vol.72 (1), p.60-68
Hauptverfasser:	He, Yuxuan, Wang, Ke, Zhao, Xingxu, Zhang, Yong, Ma, Youji, Hu, Junjie
Format:	Artikel
Sprache:	eng
Schlagworte:	Adenosine Triphosphate - metabolism Animals Apoptosis Casein Kinase II - biosynthesis Cell Survival Cryopreservation Cryopreservation - methods Hexokinase - biosynthesis Humans Male Proteome Proteome - metabolism Proteomics Ram sperm Reactive Oxygen Species - metabolism Semen Analysis Semen Preservation - methods Sheep Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization Sperm Motility - physiology Spermatozoa - metabolism
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Beschreibung
Zusammenfassung:	In this study proteomics analysis was performed to investigate damage caused to ram sperm by the freeze-thaw process. Sperm motility, viability, reactive oxygen species (ROS) and adenosine triphosphate (ATP) content were measured to evaluate sperm quality. Compared with fresh groups, motility, viability and ATP content were all lower in freeze–thawed sperm (P
ISSN:	0011-2240 1090-2392
DOI:	10.1016/j.cryobiol.2015.11.003