Study on the Possibility of Cell Growth in Tissue Culture Medium Containing Artificial Saliva

The salivary milieu should be considered when examining the biological effects of dental biomaterials. However, some human or artificial saliva components may be unsuitable for cell culture medium. The objective of the present study was to obtain basic data to develop a cell culture medium for a saliva model as an alternative to animal experiments, in order to facilitate the in vitro evaluation of dental biomaterials. Specifically, to examine whether cells can be cultured in tissue culture medium containing saliva factors, the recovery rates of two cell types were examined after the addition of human or artificial saliva to cell culture medium, demonstrating that human or artificial saliva should be diluted with tissue culture medium to about 50% or less.